Department of Forensic Medicine, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Department of Forensic Medicine, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey
Turk J Med Sci. 2020 Aug 26;50(5):1470-1479. doi: 10.3906/sag-1912-196.
Heroin can be detected and quantified by certain analytical methods, however, forensic professionals and criminal laboratories study for cheaper and faster detection tools. Surface-enhanced Raman spectroscopy (SERS) rises as a possible alternative tool with its widening application spectra. There are few studies regarding Raman and SERS spectra of heroin and its metabolites, which are unfortunately controversial. In this study, we compared five different surfaces in order to find out more efficient Raman-active substrate for opiate detection and rapid quantification of heroin and its metabolites in saliva.
Morphine standard material was used to identify proper surface for SERS analysis of opiates. Heroin and its metabolites (morphine, morphine-3-ß-glucuronide and 6-monoacetyl morphine) were calibrated between 50 ppb and 500 ppm and quantified on AuNRs with signal enhancement of silver colloids in saliva. Raman microscope with a 785-nm laser source was used.
Obtained results showed that heroin and its metabolites can be detected and quantified in saliva samples using a SERS-based system. Additionally, the present study revealed that synergetic effect of a specific gold nano-surface with ability controlling liquid motion and silver nanoparticles increase band numbers and intensities. Therefore, we suggest a fast, accurate and cost-effective method to detect and quantify heroin in biological fluids
某些分析方法可检测和定量海洛因，然而，法医学专业人员和犯罪实验室研究更便宜、更快的检测工具。表面增强拉曼光谱（SERS）因其广泛的应用谱而成为一种可行的替代工具。关于海洛因及其代谢物的拉曼和 SERS 光谱的研究很少，不幸的是这些研究存在争议。在这项研究中，我们比较了五种不同的表面，以找出更有效的拉曼活性基底，用于检测阿片类药物和快速定量唾液中海洛因及其代谢物。
使用吗啡标准物质来确定用于阿片类物质 SERS 分析的合适表面。海洛因及其代谢物（吗啡、吗啡-3-β-葡糖苷酸和 6-单乙酰吗啡）在 50ppb 至 500ppm 之间进行校准，并在唾液中用银胶体增强金纳米棒（AuNRs）进行定量。使用带有 785nm 激光源的 Raman 显微镜。
研究结果表明，可使用基于 SERS 的系统检测和定量唾液中的海洛因及其代谢物。此外，本研究表明，具有控制液体运动能力的特定金纳米表面与纳米银颗粒的协同作用增加了带的数量和强度。因此，我们提出了一种快速、准确且具有成本效益的方法，用于检测和定量生物液中的海洛因。
