与细胞聚（ADP-核糖）和PARP特异性聚（ADP-核糖）相关的蛋白质的纯化方法。
Methods for purification of proteins associated with cellular poly(ADP-ribose) and PARP-specific poly(ADP-ribose).
作者信息
Rood Jennifer E, Leung Anthony K L, Chang Paul
机构信息
Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
出版信息
Methods Mol Biol. 2011;780:153-64. doi: 10.1007/978-1-61779-270-0_10.
Poly(ADP-ribose) (pADPr) is a posttranslational modification that regulates protein function through two major mechanisms: covalent modification of acceptor proteins and noncovalent binding of proteins to pADPr. pADPr is synthesized by a family of enzymes called poly(ADP-ribose) polymerases (PARPs) that are themselves major targets of pADPr modification. Here, we outline two methods for the purification of pADPr-binding proteins via pADPr purification under native conditions: purification of cellular pADPr and pADPr covalently linked to specific PARPs. Together, these methods provide complementary approaches to the identification of noncovalent pADPr-protein interactions in the cell.
聚（ADP-核糖）（pADPr）是一种翻译后修饰，通过两种主要机制调节蛋白质功能：受体蛋白的共价修饰以及蛋白质与pADPr的非共价结合。pADPr由一类称为聚（ADP-核糖）聚合酶（PARPs）的酶合成，而这些酶本身就是pADPr修饰的主要靶点。在这里，我们概述了两种在天然条件下通过pADPr纯化来纯化pADPr结合蛋白的方法：细胞pADPr的纯化以及与特定PARPs共价连接的pADPr的纯化。总之，这些方法为鉴定细胞中非共价pADPr-蛋白质相互作用提供了互补的途径。
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