Medcalf E A, Newman D J, Gilboa A, Gorman E G, Price C P
Department of Clinical Biochemistry, London Hospital Medical College, Whitechapel, U.K.
J Immunol Methods. 1990 May 8;129(1):97-103. doi: 10.1016/0022-1759(90)90425-u.
A rapid particle-enhanced turbidimetric immunoassay (PETIA), for the measurement of serum beta 2-microglobulin is described. The method has a working range of 0.2-40 mg/l, with good precision and a correlation coefficient of 0.97 when compared with an established radioimmunoassay method. One of the major advantages of this assay is the stability of the calibration curve (up to at least 20 months). This, and the fact that no pretreatment of serum samples is necessary, makes the assay ideally suited for all types of routine determination.
本文描述了一种用于测定血清β2-微球蛋白的快速颗粒增强比浊免疫分析法(PETIA)。该方法的工作范围为0.2 - 40mg/l,精密度良好,与既定的放射免疫分析法相比,相关系数为0.97。该分析方法的主要优点之一是校准曲线的稳定性(至少可达20个月)。这一点以及血清样本无需预处理的事实,使得该分析方法非常适合所有类型的常规检测。