血清中性激素结合球蛋白的颗粒增强比浊免疫测定法。

Particle-enhanced turbidimetric immunoassay of sex-hormone-binding globulin in serum.

作者信息

Deleo D T, Lee I R, Wetherall J D, Newman D J, Medcalf E A, Price C P

机构信息

School of Biomedical Sciences, Curtin University of Technology, Bentley, Western Australia.

出版信息

Clin Chem. 1991 Apr;37(4):527-31.

PMID:2015665

Abstract

A particle-enhanced turbidimetric immunoassay (PETIA) for human sex-hormone-binding globulin (SHBG) is described. The method involves use of antibody covalently coupled to latex particles and is almost fully automated, with sample processing being complete in less than 20 min. The working reagents are stable for at least three months, and full calibration of the assay each day is not essential. A particular advantage is that pretreatment of samples is rarely required because the working range of the assay is from 2.0 to 320 nmol/L for nondiluted serum. Intra- and interassay CVs were less than 4.5% and 8.5%, respectively, and mean analytical recovery was 101.5%. SHBG concentrations of 129 serum samples determined by this method and by a commercially available immunoradiometric assay correlated highly.

摘要

本文描述了一种用于检测人性激素结合球蛋白（SHBG）的颗粒增强比浊免疫分析法（PETIA）。该方法采用与乳胶颗粒共价偶联的抗体，几乎完全自动化，样品处理在不到20分钟内即可完成。工作试剂至少稳定三个月，无需每天对检测进行全面校准。一个特别的优点是，由于该检测对未稀释血清的工作范围为2.0至320 nmol/L，因此很少需要对样品进行预处理。批内和批间变异系数分别小于4.5%和8.5%，平均分析回收率为101.5%。用该方法和市售免疫放射分析法测定的129份血清样品的SHBG浓度具有高度相关性。