State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
Vector Borne Zoonotic Dis. 2011 Jul;11(7):877-82.
Thirty-two strains of Borrelia burgdorferi sensu lato were isolated from Ixodes persulcatus ticks collected from northeastern China from May to June in 2004 and 2005. Restriction fragment length polymorphism (RFLP) analysis and sequence analysis of 5S-23S rRNA intergenic spacer revealed that 29 (90.6%) belonged to Borrelia garinii, demonstrating B, C, and a unique pattern. The remaining three isolates (9.4%) were Borrelia afzelii with pattern D. The phylogenetic analysis based on 5S-23S rRNA intergenic spacer showed that B. garinii and B. afzelii genospecies clustered into two separate lineages. B. garinii strains were classified into three different branches: All the strains with RFLP pattern C were in the same branch, strain VH10 with a unique RFLP pattern clustered with strains VH9 and MDH2 with pattern B, and the rest of the strains with pattern B constitute another branch. These findings demonstrate the genetic diversity of B. burgdorferi sensu lato isolates from northeastern China.
2004 年 5 月至 6 月，从中国东北地区采集的硬蜱中分离到 32 株博氏疏螺旋体。限制片段长度多态性（RFLP）分析和 5S-23S rRNA 基因间隔区序列分析显示，29 株（90.6%）属于伯氏疏螺旋体，表现为 B、C 和独特的模式。其余 3 株（9.4%）为模式 D 的阿费尔疏螺旋体。基于 5S-23S rRNA 基因间隔区的系统发育分析表明，伯氏疏螺旋体和阿费尔疏螺旋体种聚为两个不同的分支。伯氏疏螺旋体菌株分为 3 个不同的分支：所有具有 RFLP 模式 C 的菌株都在同一分支中，具有独特 RFLP 模式的 VH10 菌株与具有模式 B 的 VH9 和 MDH2 菌株聚类，其余具有模式 B 的菌株构成另一个分支。这些发现表明中国东北地区博氏疏螺旋体的遗传多样性。
