Kitazawa S, Maeda S, Sugiyama T
2nd Department of Pathology, Kobe University, School of Medicine, Japan.
Med Oncol Tumor Pharmacother. 1990;7(1):35-41. doi: 10.1007/BF03000489.
We raised monoclonal antibody (MAb) against a synthetic oligopeptide corresponding to a portion of the predicted v-abl protein sequence (379-390). This MAb reacted with all of the abl-gene products (p145c-abl, p150c-abl and p210bcr-abl fused protein) and was not specific for any one of them. Immunocytochemically, we investigated the expression and localization of the abl-gene products in various leukemic cell lines. Positive immunoreactions were observed in Ph1 positive leukemic cell lines (K562 and KU-812) and erythro-leukemic cell lines (HEL and K3D) and were located on the cell membrane. Electron microscopically, a different distribution pattern was observed among the cell lines: linear and almost even in Ph1 positive leukemic cell lines, whereas spotted or budding-like in erythroleukemic cell lines. Ph1 translocation produces p210bcr-abl fused protein with not only altered autophosphorylation activities but also altered subcellular distribution patterns.
我们制备了针对与预测的v-abl蛋白序列（379-390）一部分相对应的合成寡肽的单克隆抗体（MAb）。该单克隆抗体与所有abl基因产物（p145c-abl、p150c-abl和p210bcr-abl融合蛋白）发生反应，对其中任何一种都不具有特异性。通过免疫细胞化学方法，我们研究了abl基因产物在各种白血病细胞系中的表达和定位。在Ph1阳性白血病细胞系（K562和KU-812）以及红白血病细胞系（HEL和K3D）中观察到阳性免疫反应，且位于细胞膜上。在电子显微镜下，各细胞系中观察到不同的分布模式：在Ph1阳性白血病细胞系中呈线性且几乎均匀分布，而在红白血病细胞系中呈斑点状或芽状分布。Ph1易位产生的p210bcr-abl融合蛋白不仅具有改变的自身磷酸化活性，而且具有改变的亚细胞分布模式。
