Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China.
Talanta. 2011 Sep 30;85(4):2100-5. doi: 10.1016/j.talanta.2011.07.061. Epub 2011 Jul 23.
The novel molecularly imprinted microsphere (MIM) that could be applied as special sorbent was synthesized by aqueous suspension polymerization using 1,1-bis(4-chlorophenyl)-1,2,2,2-tetrachloroethane (α-chloro-DDT) as the dummy template. The obtained MIM exhibited good recognition and selectivity to dicofol and it was successfully applied as selective sorbent of solid-phase extraction for the determination of dicofol from aquatic products. At the optimum conditions of the molecularly imprinted solid-phase extraction (MISPE) coupled with GC-ECD, good linearity for dicofol was achieved in a range of 0.4-100 ng g(-1) (r(2)=0.9995) and the recoveries at three spiked levels were ranged from 85.8% to 101.2% for aquatic products with the relative standard deviation (RSD) less than 5.6%. The presented MISPE-GC-ECD method exhibited the advantages of simplicity, selectivity and sensitivity, and could be potentially applied to the determination of dicofol in complicated aquatic products.
新型分子印迹微球（MIM）可以作为特殊的吸附剂，通过水悬浮聚合反应合成，使用 1,1-双（4-氯苯基）-1,2,2,2-四氯乙烷（α-氯-DDT）作为虚拟模板。所得的 MIM 对三氯杀螨醇表现出良好的识别和选择性，并且已成功用作固相萃取的选择性吸附剂，用于从水产品中测定三氯杀螨醇。在分子印迹固相萃取（MISPE）与 GC-ECD 联用的最佳条件下，三氯杀螨醇在 0.4-100ng g(-1)（r(2)=0.9995）范围内呈现良好的线性关系，对于水产品的三个加标水平的回收率在 85.8%至 101.2%之间，相对标准偏差（RSD）小于 5.6%。所提出的 MISPE-GC-ECD 方法具有简单、选择性和灵敏性的优点，可潜在应用于复杂水产品中三氯杀螨醇的测定。
