Faculty of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Bailrigg, Lancaster LA1 4YQ, UK.
Carbohydr Res. 2011 Oct 18;346(14):2222-7. doi: 10.1016/j.carres.2011.06.033. Epub 2011 Jul 2.
The polymers chondroitin sulphate and dermatan sulphate have been fragmented by an anhydrous hydrazine/nitrous acid procedure. The resulting disaccharides from the polymer repeat sequences were reduced with NaBH(4) and purified by ion exchange chromatography. Whereas enzymatic depolymerisation leads to the loss of the distinction between glucuronic and iduronic acids of CS and DS in the resultant disaccharides, nitrous acid depolymerisation retains these structures. Complete (1)H and (13)C NMR data have been derived for the major components which were shown to have the structures: GlcA-(β1→3)-anTal6S-ol (I) and L-IdoA-(α1→3)-anTal4S-ol (II), where anTal-ol represents (2,5)anhydro-D-talitol and 6S/4S represent O-ester sulphate groups at C-6/C-4 sites.
聚合物硫酸软骨素和硫酸皮肤素已通过无水肼/亚硝酸处理进行了片段化。从聚合物重复序列中得到的二糖用 NaBH(4)还原，并通过离子交换层析进行纯化。虽然酶解会导致 CS 和 DS 中二糖中葡萄糖醛酸和艾杜糖醛酸的区别丧失，但亚硝酸解保留了这些结构。主要成分的完整 (1)H 和 (13)C NMR 数据已被推导出来，这些成分被证明具有以下结构：GlcA-(β1→3)-anTal6S-ol (I) 和 L-IdoA-(α1→3)-anTal4S-ol (II)，其中 anTal-ol 代表 (2,5) 脱水-D-塔罗糖醇，6S/4S 代表 C-6/C-4 位的 O-酯硫酸基。
