通过同位素稀释质谱法同时定量多种胆固醇自氧化和单羟基化产物。

Simultaneous quantification of several cholesterol autoxidation and monohydroxylation products by isotope-dilution mass spectrometry.

作者信息

Breuer O, Björkhem I

机构信息

Karolinska Institute, Department of Clinical Chemistry I, Huddinge University Hospital, Sweden.

出版信息

Steroids. 1990 Apr;55(4):185-92. doi: 10.1016/0039-128x(90)90109-o.

DOI:10.1016/0039-128x(90)90109-o

PMID:2187286

Abstract

An assay based on isotope-dilution mass spectrometry with deuterium-labeled internal standards was developed for simultaneous quantification of cholest-5-ene-3 beta,7 alpha-diol (7 alpha-hydroxycholesterol), cholest-5 beta,6 beta-epoxy-3 beta-ol (cholesterol-5 beta,6 beta-epoxide), cholest-5 alpha,6 alpha-epoxy-3 beta-ol (cholesterol-5 alpha,6 alpha-epoxide), cholest-5-en-7-one-3 beta-ol (7-oxocholesterol), cholestane-3 beta,5 alpha,6 beta-triol, cholest-5-ene-3 beta,25-diol (25-hydroxycholesterol), and cholest-5-ene-3 beta,26-diol (26-hydroxycholesterol) in one single serum sample. Recovery experiments and replicate analyses showed that the assay was sufficiently sensitive, accurate, and precise. The concentrations of the listed compounds in sera from 19 healthy subjects were determined and are presented.

摘要

开发了一种基于带有氘标记内标的同位素稀释质谱法的检测方法，用于在单一血清样本中同时定量测定胆甾-5-烯-3β,7α-二醇（7α-羟基胆固醇）、胆甾-5β,6β-环氧-3β-醇（胆固醇-5β,6β-环氧化物）、胆甾-5α,6α-环氧-3β-醇（胆固醇-5α,6α-环氧化物）、胆甾-5-烯-7-酮-3β-醇（7-氧代胆固醇）、胆甾烷-3β,5α,6β-三醇、胆甾-5-烯-3β,25-二醇（25-羟基胆固醇）和胆甾-5-烯-3β,26-二醇（26-羟基胆固醇）。回收率实验和重复分析表明该检测方法具有足够的灵敏度、准确性和精密度。测定并给出了19名健康受试者血清中所列化合物的浓度。