Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK.
Environ Pollut. 2011 Dec;159(12):3777-83. doi: 10.1016/j.envpol.2011.06.043. Epub 2011 Aug 27.
The effect of cable oil concentration, nutrient amendment and bioaugmentation on cable oil component biodegradation in a pristine agricultural soil was investigated. Biodegradation potential was evaluated over 21 d by measuring cumulative CO(2) respiration on a Micro-Oxymax respirometer and (14)C-phenyldodecane mineralisation using a (14)C-respirometric assay. Cable oil concentration had a significant effect upon oil biodegradation. Microbial respiratory activity increased with increasing cable oil concentration, whereas (14)C-phenydodecane mineralisation decreased. Bioaugmentation achieved the best cable oil biodegradation performance, resulting in increases in cumulative CO(2) respiration, and maximum rates and extents of (14)C-phenyldodecane mineralisation. Generally, nutrient amendment also enhanced cable oil biodegradation, but not to the extent that degrader amendment did. Cable oil biodegradation was a function of (i) cable oil concentration and (ii) catabolic ability of microbial populations. Bioaugmentation may enhance cable oil biodegradation, and is dependent upon composition, cell number and application of catabolic inocula to soil.
研究了电缆油浓度、营养物添加和生物增强对原始农业土壤中电缆油成分生物降解的影响。通过在 Micro-Oxymax 呼吸计上测量累积 CO2 呼吸和使用 14C-呼吸计测定法测定 14C-苯十二烷矿化作用，在 21 天内评估了生物降解潜力。电缆油浓度对油的生物降解有显著影响。微生物呼吸活性随电缆油浓度的增加而增加，而 14C-苯十二烷矿化作用则降低。生物增强实现了最佳的电缆油生物降解性能，导致累积 CO2 呼吸增加，以及 14C-苯十二烷矿化作用的最大速率和程度增加。一般来说，营养物添加也增强了电缆油的生物降解，但不如降解物添加那样显著。电缆油的生物降解是电缆油浓度和微生物种群的代谢能力的函数。生物增强可能会增强电缆油的生物降解，并且取决于接种物的组成、细胞数量和在土壤中的应用。
