Institute of Bioenergy and College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1570-1575. doi: 10.1099/ijs.0.036632-0. Epub 2011 Aug 26.
A taxonomic study was carried out on strain P1(T), which was isolated from mangrove sediment samples collected from Qinglan Port (Hainan, China). Cells were curved rods, that were motile, with a single polar flagellum. The strain was non-spore-forming with a cell size of 0.6×1.5-2.2 µm. Catalase and oxidase activities were not detected. Growth was observed in the temperature range 22-44 °C (optimum, 35-40 °C) and pH range 5.5-8.5 (optimum, pH 7.0). NaCl was required for growth and tolerated at up to 3.5% (w/v) (optimum, 0.5%). Strain P1(T) utilized hydrogen, succinate, L-malate, citrate, oxalate, DL-lactate, pyruvate, or cysteine as electron donors, and sulfate or sulfite as electron acceptors. Fermentation products from pyruvate were acetate, H(2) and CO(2). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain P1(T) formed a distinct evolutionary lineage within the family Desulfovibrionaceae. Strain P1(T) was most closely related to members of the genera Desulfovibrio (92.0-94.3% 16S rRNA gene sequence similarity), Desulfocurvus (91.1%), Bilophila (87.9%) and Lawsonia (86.0%) of the family Desulfovibrionaceae. The DNA G+C content of strain P1(T) was 64.5 mol% and the major cellular fatty acids were iso-C(15:0) (18.8%), anteiso-C(15:0) (5.0%), C(16:0) (14.2%) and iso-C(17:1)ω9c (24.4%). The predominant menaquinone was MK-7 (97%). Major polar lipids were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Strain P1(T) was distinguishable from members of phylogenetically related genera by differences in several phenotypic properties. On the basis of the phenotypic and phylogenetic data, strain P1(T) represents a novel species of a new genus, for which the name Desulfobaculum xiamenensis gen. nov., sp. nov. is proposed. The type strain of Desulfobaculum xiamenensis is P1(T) (=CGMCC 1.5166(T)=DSM 24233(T)).
进行了 P1(T)菌株的分类学研究，该菌株从中国海南青澜港的红树林沉积物样本中分离得到。细胞呈弯曲的杆状，可运动，有一个单极鞭毛。该菌株不产芽孢，细胞大小为 0.6×1.5-2.2 µm。未检测到过氧化氢酶和氧化酶活性。在 22-44°C（最佳，35-40°C）和 pH 5.5-8.5（最佳，pH 7.0）的范围内观察到生长。生长需要 NaCl，可耐受高达 3.5%（w/v）（最佳，0.5%）。P1(T)菌株利用氢、琥珀酸盐、L-苹果酸盐、柠檬酸盐、草酸盐、DL-乳酸盐、丙酮酸或半胱氨酸作为电子供体，硫酸盐或亚硫酸盐作为电子受体。丙酮酸的发酵产物为乙酸、H(2)和 CO(2)。基于 16S rRNA 基因序列的系统发育分析表明，P1(T)菌株在脱硫弧菌科内形成了一个独特的进化谱系。P1(T)菌株与脱硫弧菌属（92.0-94.3% 16S rRNA 基因序列相似性）、脱硫弯杆菌属（91.1%）、拟杆菌属（87.9%）和劳森氏菌属（86.0%）的成员最为密切相关。P1(T)菌株的 DNA G+C 含量为 64.5 mol%，主要细胞脂肪酸为 iso-C(15:0)（18.8%）、anteiso-C(15:0)（5.0%）、C(16:0)（14.2%）和 iso-C(17:1)ω9c（24.4%）。主要的menaquinone 是 MK-7（97%）。主要的极性脂为磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰甘油。P1(T)菌株在几个表型特性上与亲缘关系较近的属成员有所不同。基于表型和系统发育数据，P1(T)菌株代表了一个新属的新种，提议将其命名为厦门脱硫杆菌（Desulfobaculum xiamenensis）属，新种。厦门脱硫杆菌的模式菌株为 P1(T)（=CGMCC 1.5166(T)=DSM 24233(T)）。
