Department of Animal Science, University of Wyoming, Laramie, Wyoming 82071, USA.
J Anim Sci. 2012 Jan;90(1):325-33. doi: 10.2527/jas.2011-4155. Epub 2011 Aug 26.
Many areas of the western United States have soils that have increased Se content, and ruminants grazing these rangelands may ingest increased quantities of Se. In addition, high-energy diets or increased Se intake may induce gut inflammation. The objective of this study was to evaluate the effects of maternal plane of nutrition and increased dietary Se during gestation on inflammatory responses in neonatal lamb ileal tissue, a major immune organ. Rambouillet ewes (age = 240 ± 17 d; initial BW = 52.1 ± 6.2 kg) were allocated to 4 treatments arranged in a 2 × 2 factorial. Factors included Se [adequate Se (ASe, 11.5 µg/kg of BW) or high Se (HSe, 77.0 µg/kg of BW)] initiated at breeding, and nutritional plane [100% (CON) or 140% (HIH) of requirements] initiated at d 40 of gestation. Ewes were fed individually from d 40, and lambs were removed at parturition and fed artificial colostrum and milk replacer. Lambs were necropsied at 20 d of age, and ileal tissues were sampled for immunoblotting and real-time quantitative reverse-transcription PCR analyses. The ASe-HIH and HSe-CON treatments had no effect (P = 0.179) on inflammatory signaling compared with ASe-CON. However, greater inflammatory signaling was detected in the HSe-HIH group, as shown by increased (P < 0.05) mRNA expression of tumor necrosis factor-α and chemotaxis IL-8. Consistently, phosphorylation of c-Jun N-terminal kinase, a primary inflammatory signaling mediator, was greater (P < 0.05) in the HSe-HIH group compared with other treatments. Consistent with cytokine expression, mast cell density was less in the HSe-CON group than in other treatments. The expression of transforming growth factor β mRNA was greater (P < 0.05) in the HSe-HIH group; consistently, collagen content was increased in the HSe-HIH group compared with the ASe-CON group (P < 0.05). In conclusion, independently, neither HSe nor HIH had major effects on inflammation, but in combination, these maternal treatments induced an inflammatory response in the neonatal intestine.
美国西部的许多地区的土壤中硒含量增加，而在这些牧场放牧的反刍动物可能会摄入更多的硒。此外，高能饮食或增加硒的摄入可能会引起肠道炎症。本研究的目的是评估母体营养水平和妊娠期间增加的饮食硒对新生羔羊回肠组织炎症反应的影响，回肠是主要的免疫器官。兰布赖特母羊（年龄=240±17d；初始 BW=52.1±6.2kg）被分配到 4 个处理中，采用 2×2 析因设计。因素包括硒[适量硒（ASe，11.5μg/kg BW）或高硒（HSe，77.0μg/kg BW）]于配种时开始，以及营养水平[妊娠第 40 天 100%（CON）或 140%（HIH）]开始。母羊从第 40 天开始单独喂养，羔羊在分娩时被取出并喂食人工初乳和代乳料。羔羊在 20 日龄时进行剖检，回肠组织用于免疫印迹和实时定量逆转录 PCR 分析。与 ASe-CON 相比，ASe-HIH 和 HSe-CON 处理对炎症信号没有影响（P=0.179）。然而，在 HSe-HIH 组中检测到更高的炎症信号，表现为肿瘤坏死因子-α和趋化因子 IL-8 的 mRNA 表达增加（P<0.05）。同样，初级炎症信号介质 c-Jun N-末端激酶的磷酸化在 HSe-HIH 组中也更高（P<0.05）。与细胞因子表达一致，HSe-CON 组的肥大细胞密度低于其他处理组。转化生长因子-β mRNA 的表达在 HSe-HIH 组中更高（P<0.05）；同样，与 ASe-CON 组相比，HSe-HIH 组的胶原含量增加（P<0.05）。总之，单独的 HSe 或 HIH 对炎症没有重大影响，但联合起来，这些母体处理会在新生动物的肠道中引起炎症反应。
