微小RNA-149促进鼻咽癌细胞的上皮-间质转化和侵袭。
miR-149 promotes epithelial-mesenchymal transition and invasion in nasopharyngeal carcinoma cells.
作者信息
Luo Zhaohui, Zhang Liyang, Li Zheng, Jiang Chen, Dai Yafei, Liu Xiaoping, Zheng Ying, Yu Haibo, Xiang Juanjuan, Li Guiyuan
机构信息
Cancer Research Institute, Central South University, Changsha 410078, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Jul;36(7):604-9. doi: 10.3969/j.issn.1672-7347.2011.07.004.
OBJECTIVE
To investigate the function and mechanism of miR-149 in nasopharyngeal carcinoma (NPC).
METHODS
The expression of miR-149 was examined by real-time PCR and calculated by 2(-▵▵Ct) method. The cell proliferation was analyzed by MTT assay. The cell migration and invasion were shown by the wound healing assay and transwell migration assay, and the expression of E-cadherin was detected by Western blot.
RESULTS
The expression of miR-149 was higher in NPC cell lines 5-8F and 6-10B than that in normal immortalized nasopharyngeal epithelial NP69. MTT assay showed that miR-149 promoted the proliferation of NPC cell lines. The wound healing assay showed miR-149 promoted the mobility and invasion of NPC cell lines. Inhibition of miR-149 reduced the ability of NPC cell lines to proliferate and invade. miR-149 downregulated the expression of E-cadherin, whereas antagomir which mediated knockdown of miR-149 significantly upregulated the expression of E-cadherin.
CONCLUSION
miR-149 might be involved in the invasion and metastasis of NPC through regulation of epithelial-mesenchymal transition (EMT).
目的
探讨miR-149在鼻咽癌（NPC）中的作用及机制。
方法
采用实时定量PCR检测miR-149的表达，并通过2(-▵▵Ct)法计算。采用MTT法分析细胞增殖情况。通过伤口愈合实验和Transwell迁移实验观察细胞迁移和侵袭能力，并采用蛋白质免疫印迹法检测E-钙黏蛋白的表达。
结果
miR-149在NPC细胞系5-8F和6-10B中的表达高于正常永生化鼻咽上皮细胞NP69。MTT实验表明miR-149促进NPC细胞系的增殖。伤口愈合实验表明miR-149促进NPC细胞系的迁移和侵袭。抑制miR-149可降低NPC细胞系的增殖和侵袭能力。miR-149下调E-钙黏蛋白的表达，而介导miR-149敲低的拮抗剂显著上调E-钙黏蛋白的表达。
结论
miR-149可能通过调节上皮-间质转化（EMT）参与NPC的侵袭和转移。
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