Jin Lu, Li Yifan, Zhang Zeng, He Tao, Hu Jia, Liu Jiaju, Chen Mingwei, Gui Yaoting, Yang Shangqi, Mao Xiangming, Chen Yun, Lai Yongqing
Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
Department of Urology, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Oncol Lett. 2017 Nov;14(5):5624-5630. doi: 10.3892/ol.2017.6855. Epub 2017 Aug 31.
Renal cell carcinoma (RCC) is the most common type of kidney cancer, and the prognosis of metastatic RCC remains poor with a high rate of recurrence and mortality. A previous study has revealed that microRNA (miRNA), which negatively regulates protein expression, serves a role of oncogene or tumor suppressor. The aim of the present study was to investigate the expression and function of miR-514a-3p in RCC. To detect the expression of miR-514a-3p in 32 paired RCC tissues, quantitative polymerase chain reaction (qPCR) was performed. The function of miR-514a-3p in the proliferation, mobility and apoptosis of RCC cells (786-O and ACHN) was assessed by MTT, CCK-8, cell scratch, Transwell, Hoechst 33342 staining and flow cytometry assay. The results of qPCR revealed that miR-514a-3p was significantly downregulated in RCC tissues compared with adjacent normal tissues. Upregulation of miR-514a-3p by transfection of mimics suppressed RCC cell proliferation, migration and invasion, and induced cell apoptosis. The results revealed that miR-514a-3p was significantly downregulated in RCC and may serve a role as tumor suppressor in RCC. Further studies are required, focusing on the possibility of using miR-514a-3p as a biomarker for RCC as well as the pathway of miR-514a-3p in RCC.
肾细胞癌（RCC）是最常见的肾癌类型，转移性RCC的预后仍然很差，复发率和死亡率很高。先前的一项研究表明，负向调节蛋白质表达的微小RNA（miRNA）发挥癌基因或肿瘤抑制因子的作用。本研究的目的是调查miR-514a-3p在RCC中的表达和功能。为检测32对RCC组织中miR-514a-3p的表达，进行了定量聚合酶链反应（qPCR）。通过MTT、CCK-8、细胞划痕、Transwell、Hoechst 33342染色和流式细胞术检测评估miR-514a-3p在RCC细胞（786-O和ACHN）增殖、迁移和凋亡中的作用。qPCR结果显示，与相邻正常组织相比，RCC组织中miR-514a-3p明显下调。通过转染模拟物上调miR-514a-3p可抑制RCC细胞增殖、迁移和侵袭，并诱导细胞凋亡。结果表明，miR-514a-3p在RCC中明显下调，可能在RCC中发挥肿瘤抑制因子的作用。需要进一步研究，重点关注将miR-514a-3p用作RCC生物标志物的可能性以及miR-514a-3p在RCC中的作用途径。
