Molecular Genetics of Industrial Microorganisms, Wageningen University, Wageningen, The Netherlands.
Appl Microbiol Biotechnol. 2012 Jan;93(1):285-93. doi: 10.1007/s00253-011-3550-6. Epub 2011 Aug 27.
AmyR is commonly considered a regulator of starch degradation whose activity is induced by the presence of maltose, the disaccharide building block of starch. In this study, we demonstrate that the role of AmyR extends beyond starch degradation. Enzyme activity assays, genes expression analysis and growth profiling on D-glucose- and D-galactose-containing oligo- and polysaccharides showed that AmyR regulates the expression of some of the Aspergillus niger genes encoding α- and β-glucosidases, α- and β- galactosidases, as well as genes encoding α-amlyases and glucoamylases. In addition, we provide evidence that D-glucose or a metabolic product thereof may be the inducer of the AmyR system in A. niger and not maltose, as is commonly assumed.
AmyR 通常被认为是淀粉降解的调节剂，其活性可被麦芽糖诱导，麦芽糖是淀粉的二糖组成部分。在本研究中，我们证明了 AmyR 的作用超出了淀粉降解的范围。酶活性测定、基因表达分析以及在含有 D-葡萄糖和 D-半乳糖的寡糖和多糖上的生长分析表明，AmyR 调节了一些黑曲霉基因的表达，这些基因编码α-和β-葡萄糖苷酶、α-和β-半乳糖苷酶，以及编码α-淀粉酶和葡萄糖淀粉酶的基因。此外，我们提供的证据表明，D-葡萄糖或其代谢产物可能是黑曲霉中 AmyR 系统的诱导物，而不是通常假设的麦芽糖。
