Garrigues Sandra, Kun Roland S, Peng Mao, Bauer Diane, Keymanesh Keykhosrow, Lipzen Anna, Ng Vivian, Grigoriev Igor V, de Vries Ronald P
Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands.
USA Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA.
iScience. 2022 Mar 12;25(4):104065. doi: 10.1016/j.isci.2022.104065. eCollection 2022 Apr 15.
Efficient utilization of agro-industrial waste, such as sugar beet pulp, is crucial for the bio-based economy. The fungus possesses a wide array of enzymes that degrade complex plant biomass substrates, and several regulators have been reported to play a role in their production. The role of the regulators GaaR, AraR, and RhaR in sugar beet pectin degradation has previously been reported. However, genetic regulation of the degradation of sugar beet pulp has not been assessed in detail. In this study, we generated a set of single and combinatorial deletion mutants targeting the pectinolytic regulators GaaR, AraR, RhaR, and GalX as well as the (hemi-)cellulolytic regulators XlnR and ClrB to address their relative contribution to the utilization of sugar beet pulp. We show that has a flexible regulatory network, adapting to the utilization of (hemi-)cellulose at early timepoints when pectin degradation is impaired.
高效利用农业工业废弃物，如甜菜浆，对生物基经济至关重要。该真菌拥有多种可降解复杂植物生物质底物的酶，并且据报道有几种调节因子在这些酶的产生中发挥作用。此前已有报道调节因子GaaR、AraR和RhaR在甜菜果胶降解中的作用。然而，尚未对甜菜浆降解的遗传调控进行详细评估。在本研究中，我们构建了一组针对果胶分解调节因子GaaR、AraR、RhaR和GalX以及（半）纤维素分解调节因子XlnR和ClrB的单基因缺失突变体和组合缺失突变体，以研究它们对甜菜浆利用的相对贡献。我们表明，当果胶降解受损时，该真菌具有灵活的调控网络，能够在早期适应（半）纤维素的利用。
