人内皮细胞单层中内皮通透性和白细胞迁移的评估。

Assessment of endothelial permeability and leukocyte transmigration in human endothelial cell monolayers.

作者信息

Ludwig Andreas, Sommer Anselm, Uhlig Stefan

机构信息

Institute of Pharmacology and Toxicology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany.

出版信息

Methods Mol Biol. 2011;763:319-32. doi: 10.1007/978-1-61779-191-8_22.

DOI:10.1007/978-1-61779-191-8_22

PMID:21874462

Abstract

Increased vascular permeability is the hallmark of inflammation. Here, we describe three methods to assess vascular permeability in cell culture: (1) Impedance measurements of endothelial cell monolayers that allow to monitor changes in cell shape in real time. (2) Diffusion of fluorescently labeled dextran across endothelial cell monolayers to identify openings large enough for bulky molecules. (3) Transmigration of neutrophils through confluent endothelial cell monolayers to study one major process that increases endothelial permeability in inflammation.

摘要

血管通透性增加是炎症的标志。在此，我们描述三种在细胞培养中评估血管通透性的方法：（1）对内皮细胞单层进行阻抗测量，可实时监测细胞形状的变化。（2）荧光标记的葡聚糖在内皮细胞单层中的扩散，以识别足以让大分子通过的开口。（3）中性粒细胞通过汇合的内皮细胞单层进行迁移，以研究炎症中增加内皮通透性的一个主要过程。