Garcia Alexander N, Vogel Stephen M, Komarova Yulia A, Malik Asrar B
Department of Pharmacology, University of Illinois, Chicago, IL, USA.
Methods Mol Biol. 2011;763:333-54. doi: 10.1007/978-1-61779-191-8_23.
The methods for assessment of endothelial barrier permeability are vital tools of experimental biology. They allow us to measure permeability of endothelial monolayer in cell culture and in lung vessels or to determine formation of tissue edema resulting from increased permeability of vasculature. This chapter provides an overview of the most common protocols.
评估内皮屏障通透性的方法是实验生物学的重要工具。它们使我们能够测量细胞培养物中以及肺血管中内皮单层的通透性,或者确定由于血管系统通透性增加而导致的组织水肿的形成。本章概述了最常见的实验方案。