Lee Jisook, Baird Andrew, Eliceiri Brian P
Department of Surgery, University of California San Diego, San Diego, CA, USA.
Methods Mol Biol. 2011;763:417-22. doi: 10.1007/978-1-61779-191-8_28.
The normal blood-brain barrier (BBB) consists of tight interendothelial cell junctions and adjacent astrocyte end feet separated by a basal lamina surrounding the endothelium. The interactions between the different cell types of BBB are disrupted in distinct patterns in the microenvironment of glioma. Malignant gliomas infiltrate the surrounding normal brain parenchyma; a process associated with vascular permeability (VP) and breakdown of the BBB. Herein, we describe methods to quantitatively measure glioma-induced vascular permeability, utilizing an orthotopic xenograft model of glioma.
正常的血脑屏障（BBB）由紧密的内皮细胞间连接和相邻的星形胶质细胞终足组成，二者被围绕内皮的基膜分隔开。在胶质瘤的微环境中，血脑屏障不同细胞类型之间的相互作用以不同模式被破坏。恶性胶质瘤浸润周围正常脑实质；这一过程与血管通透性（VP）及血脑屏障的破坏有关。在此，我们描述了利用胶质瘤原位异种移植模型定量测量胶质瘤诱导的血管通透性的方法。
