Cancer models-multiparametric applications of clinical MRI in rodent hepatic tumor model.

Small animal imaging has been a major player in an increasing amount of oncological experiments wherein magnetic resonance imaging (MRI) has become a favorite choice of measures for in vivo small animal imaging due to its advantages of excellent resolution and innocuousness. Based on a clinical MRI scanner, we propose a protocol of multiparametric MRI for noninvasive characterization and therapeutic evaluation of a rat model with implanted liver tumors. This protocol contains six sequences, namely, T (1)-weighted image (T1WI), T (2)-weighted image (T2WI), diffusion-weighed imaging (DWI), T (1)-weighted dynamic contrast-enhanced MRI (DCE-MRI), T (2)-weighted dynamic susceptibility contrast-enhanced MRI (DSC-MRI), and contrast-enhanced T1WI (CE-T1WI), for acquiring anatomic, diffusion, and perfusion information of tumor models. In this chapter, the details about this complete MRI protocol and the rodent liver tumor model are described in order to facilitate the readers to perform their own translational animal imaging research.