Ziegler Alexander, Mueller Susanne
Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138, USA.
Methods Mol Biol. 2011;771:633-51. doi: 10.1007/978-1-61779-219-9_32.
Magnetic resonance imaging (MRI) is now considered a routine tool for comparative morphological imaging in small vertebrate model organisms. However, the application of high-resolution imaging protocols to visualize the anatomy of invertebrate organisms has not yet become a generally accepted tool among zoologists. Here, we describe MRI protocols that permit visualization of both the internal and the external anatomy of freshly fixed invertebrates and specimens from museum collections. The choice of protocols has been optimized to allow the assembly of the large numbers of datasets that are necessary for comparative morphological analyses. Although the primary focus of our work is on sea urchin internal anatomy, we also present results from a variety of other invertebrate taxa to demonstrate the principal feasibility of MRI studies to obtain anatomical information at high resolutions. Furthermore, we briefly describe procedures suitable for 3D modelling.
磁共振成像（MRI）现在被认为是小型脊椎动物模型生物比较形态学成像的常规工具。然而，应用高分辨率成像方案来可视化无脊椎动物的解剖结构尚未成为动物学家普遍接受的工具。在这里，我们描述了MRI方案，这些方案允许可视化新鲜固定的无脊椎动物和博物馆收藏标本的内部和外部解剖结构。方案的选择已经过优化，以允许组装比较形态学分析所需的大量数据集。虽然我们工作的主要重点是海胆的内部解剖结构，但我们也展示了来自各种其他无脊椎动物类群的结果，以证明MRI研究在高分辨率下获取解剖信息的主要可行性。此外，我们简要描述了适用于三维建模的程序。
