ZomerBloemen BV, 7 Dalweg, 3707BE, Zeist, The Netherlands.
Anal Bioanal Chem. 2011 Nov;401(9):2945-54. doi: 10.1007/s00216-011-5342-3. Epub 2011 Aug 28.
Acridinium esters traditionally are triggered using basic hydrogen peroxide. By serendipity, we have found that acridinium esters can also be triggered with emission of chemiluminescence by reductive triggering, e.g., by zinc metal or reduced forms of ferric and cupric salts. Furthermore, organic reducing compounds like dithiothreitol, tricarboxyethylphosphine or glutathione could be used in combination with organic oxidants like quinones or inorganic ferric or cupric salts. Mechanisms are proposed which involve the intermediacy of superoxide. Two forms of reactive oxygen species (i.e., hydrogen peroxide and superoxide) could be discriminated based on differences in kinetics. Some applications (improved detection of acridinium ester, use of acridinium ester as redox probes) are discussed.
吖啶酯传统上是用碱性过氧化氢引发的。偶然发现，吖啶酯也可以通过还原引发来触发化学发光，例如用锌金属或三价铁和铜盐的还原形式。此外，有机还原剂，如二硫苏糖醇、三羧乙基膦或谷胱甘肽，可以与醌类或无机铁盐或铜盐等有机氧化剂结合使用。提出了涉及超氧化物中间产物的机制。两种形式的活性氧（即过氧化氢和超氧化物）可以基于动力学差异进行区分。讨论了一些应用（吖啶酯检测的改进、吖啶酯作为氧化还原探针的使用）。
