Brown Richard C, Li Zhaoqiang, Rutter Andrew J, Mu Xiaojing, Weeks Owen H, Smith Keith, Weeks Ian
Molecular Light Technology Research Limited, (Subsidiary of Gen-Probe Incorporated), 10210 Genetic Center Drive, San Diego, CA 92121, USA.
Org Biomol Chem. 2009 Jan 21;7(2):386-94. doi: 10.1039/b811947c. Epub 2008 Nov 24.
Chemiluminescent acridinium esters (AEs) permit the development of high sensitivity ligand binding assays due to a combination of high intensity light emission and very low backgrounds. Here these advantages are exploited for use in homogeneous nucleic acid hybridisation assays using quenched chemiluminescence. AE chemiluminescence is conventionally initiated at highly alkaline pH. Novel "active" AEs were designed that permit initiation under conditions compatible with maintenance of nucleic acid hybrids (i.e. pH less than 9). Methyl red was found to be a dark quencher species capable of functioning at this pH. Practical application of the chemiluminescence quenching assay system has been demonstrated using two model nucleic acid hybridisation assays based on intra- and intermolecular emitter/quencher pairs.
化学发光吖啶酯（AEs）由于其高强度发光和极低的背景相结合，使得高灵敏度配体结合分析得以发展。在此，利用这些优势开发了基于猝灭化学发光的均相核酸杂交分析方法。传统上，AE化学发光是在高碱性pH条件下引发的。设计了新型“活性”AEs，使其能够在与维持核酸杂交相兼容的条件下（即pH小于9）引发反应。发现甲基红是一种能够在该pH下发挥作用的暗猝灭剂。基于分子内和分子间发射体/猝灭剂对的两种模型核酸杂交分析方法已证明了化学发光猝灭分析系统的实际应用。
