Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Alexandria University, El-Khartoom Square, Azarita, Alexandria, Egypt.
Microb Drug Resist. 2011 Dec;17(4):489-95. doi: 10.1089/mdr.2010.0195. Epub 2011 Aug 29.
The emergence of infections caused by multidrug-resistant Gram-negative bacteria, in particular Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae, has necessitated the search for alternative therapy by either introducing new agents or renewing interest in old agents. This study compares the in vitro activity of tigecycline (TIG), recently introduced to Egyptian market, to other potentially active antimicrobials as Colistin (COL), imipenem (IPM), levofloxacin (LEV), and piperacillin/tazobactam (PIP/TAZ) against 67 Gram-negative clinical isolates obtained from El- Meery Hospital in Alexandria, Egypt. El-Meery Hospital is a 1,500-bed tertiary teaching hospital where TIG has not been previously used. Based on MIC(90)s, TIG was found to be a comparator to IPM and COL (MIC(90)= 8 μg/ml). LEV and PIP/TAZ were less active than TIG exhibiting high MIC(90)s. TIG inhibited 100% of Escherichia coli and K. pneumoniae and 60% of Ps. aeruginosa and A. baumannii isolates. In time-kill studies against IPM-resistant isolates, TIG showed bactericidal activity after 6 hours of contact against the Enterobacteriaceae isolates and after 3 hours for the tested Ps. aeruginosa isolates at 4× and 8× MIC. Against A. baumannii, TIG exerted a bacteriostatic effect. TIG demonstrated variable ability to suppress biofilm formation affecting mainly E. coli and A. baumannii isolates. These results point TIG to be a promising agent in treatment of infections caused by strains for which adequate therapy has been limited. As far as we know, this is the first report evaluating the in vitro activity of TIG against Egyptian clinical isolates.
多药耐药革兰氏阴性菌（尤其是铜绿假单胞菌、鲍曼不动杆菌和肺炎克雷伯菌）引起的感染的出现，使得人们不得不寻找新的治疗方法，或者重新关注老的治疗方法。本研究比较了替加环素（TIG）——最近引入埃及市场——与其他潜在有效的抗菌药物（如多粘菌素（COL）、亚胺培南（IPM）、左氧氟沙星（LEV）和哌拉西林/他唑巴坦（PIP/TAZ））对 67 株从埃及亚历山大的 El-Meery 医院获得的革兰氏阴性临床分离株的体外活性。El-Meery 医院是一家拥有 1500 张床位的三级教学医院，此前尚未使用 TIG。根据 MIC(90)s，TIG 被认为与 IPM 和 COL 具有可比性（MIC(90)=8μg/ml）。LEV 和 PIP/TAZ 的活性不如 TIG，MIC(90)较高。TIG 抑制了 100%的大肠埃希菌和肺炎克雷伯菌以及 60%的铜绿假单胞菌和鲍曼不动杆菌分离株。在针对 IPM 耐药分离株的时间杀伤研究中，TIG 在接触 6 小时后对肠杆菌科分离株和接触 3 小时后对测试的铜绿假单胞菌分离株显示出杀菌活性，MIC 为 4×和 8×。对鲍曼不动杆菌，TIG 表现出抑菌作用。TIG 对生物膜形成的抑制能力存在差异，主要影响大肠埃希菌和鲍曼不动杆菌分离株。这些结果表明 TIG 是一种有前途的治疗药物，可以治疗那些治疗方法有限的菌株引起的感染。据我们所知，这是首次评估 TIG 对埃及临床分离株的体外活性的报告。
