Department of Medical and Experimental Biochemistry, University "Ss Cyril and Methodius", Skopje, Republic of Macedonia.
Bosn J Basic Med Sci. 2011 Aug;11(3):169-73. doi: 10.17305/bjbms.2011.2569.
Cholesteryl ester transfer protein (CETP) plays a key role in reverse cholesterol transport and high density lipoprotein (HDL) metabolism. Predominance of small, dense LDL particles is associated with an increased risk of atherosclerosis and coronary heart disease (CHD).The aim of the study was to determine the potential relationship between the CETP concentration and low density lipoprotein (LDL) particle size and their association with intima media thickness (IMT) in patients with CHD. Lipid parameters, CETP concentration and LDL particle size were determined in 100 healthy subjects (control group) and in 100 patients with CHD, aged 43 to 77 years. Plasma CETP concentrations were measured by an enzyme-linked immuno-sorbent assay with two different monoclonal antibodies. LDL subclasses were separated by nondenaturing polyacrilamide 3-31% gradient gel electrophoresis. CETP concentration was higher in patients compared to controls (2.02 ± 0.75 mg/ml vs. 1.74 ± 0.63 mg/ml, p<0.01). Mean LDL particle size (nm) was significantly smaller in patients than in controls (24.5 ± 1.1 vs. 26.1 ± 0.9; p<0.001). There was no relation between LDL particle size and CETP concentration (r=-0.1807, p=0.072). Age, diastolic blood pressure, CETP concentration and LDL particle size were independent factors for determing IMT by multiple linear regression analysis. They accounted for 35.2 % of the observed variability in IMT. CETP is not an independent contributor of LDL particle size. CETP might play a role in determining lipoprotein distributions, but did not seem to be the sole factor in the formation of small LDL particles.
胆固醇酯转移蛋白（CETP）在胆固醇逆转运和高密度脂蛋白（HDL）代谢中起关键作用。小而密的 LDL 颗粒占优势与动脉粥样硬化和冠心病（CHD）的风险增加有关。本研究旨在确定 CETP 浓度与 LDL 颗粒大小之间的潜在关系及其与 CHD 患者内膜中层厚度（IMT）的相关性。在 100 名健康受试者（对照组）和 100 名 CHD 患者中测定了血脂参数、CETP 浓度和 LDL 颗粒大小，年龄 43 至 77 岁。使用两种不同的单克隆抗体通过酶联免疫吸附测定法测定血浆 CETP 浓度。通过非变性聚丙烯酰胺 3-31％梯度凝胶电泳分离 LDL 亚类。与对照组相比，患者的 CETP 浓度更高（2.02±0.75mg/ml 比 1.74±0.63mg/ml，p<0.01）。与对照组相比，患者的 LDL 颗粒大小（nm）明显较小（24.5±1.1nm 比 26.1±0.9nm，p<0.001）。LDL 颗粒大小与 CETP 浓度之间无相关性（r=-0.1807，p=0.072）。多元线性回归分析显示，年龄、舒张压、CETP 浓度和 LDL 颗粒大小是 IMT 的独立决定因素。它们占 IMT 观察到的变异性的 35.2％。CETP 不是 LDL 颗粒大小的独立贡献者。CETP 可能在决定脂蛋白分布中起作用，但似乎不是形成小 LDL 颗粒的唯一因素。
