Yun S H, Seo M G, Jung B Y, Kim T H, Kwon O D, Jeong K S, Rhee M H, Lee Y J, Park S J, Kwon Y S, Kwak D
College of Veterinary Medicine, Kyungpook National University, Bukgu, Daegu, Republic of Korea.
J Helminthol. 2012 Sep;86(3):363-7. doi: 10.1017/S0022149X11000496. Epub 2011 Aug 30.
While multiple DNase activities occur in the excretory/secretory products (ESPs) of the adult Haemonchus contortus, the DNase activities in ESPs of the infective larvae (L3) have not been studied. Thus, the DNase activities in ESPs of H. contortus L3 were investigated and compared to those of adults for developmental stage-specific analysis. The DNase activities had relative molecular masses (M rs) of 34 and 36 kDa upon zymographic analysis at pH 5.0 and 7.0 when the larvae were incubated for over 48 h. The 34 and 36 kDa DNases of L3 ESPs were also detected in adult ESPs with similar characteristics. However, the 37 and 38.5 kDa DNases of the adult ESPs were not detected in the L3 ESPs. Since the 37 and 38.5 kDa DNase activities were mainly detected in adult ESPs, these activities appear to be specific to the adult stage whereas the other ESP DNase activities appear to be expressed during multiple stages of the parasite's life cycle. While the difference in DNase activities of L3 and adults remains obscure, the role of DNase in larval development should be further clarified and the identification of stage-specific developmental markers will lead to the discovery of specific factors that stimulate larval development.
虽然成年捻转血矛线虫的排泄/分泌产物（ESPs）中存在多种脱氧核糖核酸酶（DNase）活性，但尚未对感染性幼虫（L3）的ESPs中的DNase活性进行研究。因此，对捻转血矛线虫L3的ESPs中的DNase活性进行了研究，并与成虫的进行比较，以进行发育阶段特异性分析。当幼虫孵育超过48小时时，在pH 5.0和7.0条件下进行酶谱分析，DNase活性的相对分子质量（Mr）为34和36 kDa。在具有相似特征的成虫ESPs中也检测到了L3 ESPs的34 kDa和36 kDa DNases。然而，在L3 ESPs中未检测到成虫ESPs的37 kDa和38.5 kDa DNases。由于37 kDa和38.5 kDa DNase活性主要在成虫ESPs中检测到，这些活性似乎是成虫阶段特有的，而其他ESPs DNase活性似乎在寄生虫生命周期的多个阶段表达。虽然L3和成虫的DNase活性差异尚不清楚，但DNase在幼虫发育中的作用应进一步阐明，鉴定阶段特异性发育标志物将有助于发现刺激幼虫发育的特定因子。
