Zou Ji-dong, Fu Hai-yan, Xu Wei, Lü Zheng-hua, Cao Hong-yuan, Luan Xin-yong
Department of Otorhinolaryngology Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Zhonghua Zhong Liu Za Zhi. 2011 Jun;33(6):461-4.
To detect lymphangiogenesis by labeling the lymphatic endothelial marker, lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), and study the prognostic relevance of lymphangiogenesis in laryngeal squamous carcinoma.
Clinical files and specimens of 78 patients with histologically diagnosed laryngeal carcinoma were stained with LYVE-1 as a specific lymphatic endothelial marker. The lymphatic vessel density (LVD) was measured, and the correlation between LVD and clinicopathological features of the tumor cases was analyzed.
The mean LVD in laryngeal carcinoma (13.24 ± 5.09) was significantly higher than that in adult laryngeal papilloma (5.54 ± 3.15) and squamous dysplasia (6.76 ± 4.45, P < 0.05). The LVD of poorly differentiated tumors (15.74 ± 5.24) was significantly higher than that in the moderately differentiated tumors (13.84 ± 6.20), and the LVD in the moderately differentiated tumors was significantly higher than that in the well-differentiated tumors (11.68 ± 6.34). The LVD in stage 0 to stage II group (10.66 ± 5.70) was significantly lower than that in the stage III to IV group (17.01 ± 6.35). The lymph node metastasis group (17.25 ± 7.37) was significantly higher than non-lymph node metastasis group (8.60 ± 5.23, P < 0.05). There was no significant association between LVD and age, sex, primary site and distant metastasis. The overall survival in the patients with a LVD higher than the mean value was 33.5 month, and that of cases with a LVD lower than the mean value was 81.6 month (P < 0.05). The multivariate survival analysis showed that the clinical stage and LVD were independent prognostic factors of laryngeal cancer.
The LYVE-1 staining histochemistry demonstrates that the lymphangiogenesis occurrs mainly at the edge of the tumors, and lymphangiogenesis plays an important role in the carcinogenesis, cancer progression and lymph node metastasis in laryngeal cancer. LVD may be an independent indicator of poor prognosis of laryngeal cancer.
通过标记淋巴管内皮标志物淋巴管内皮透明质酸受体-1（LYVE-1）来检测淋巴管生成，并研究淋巴管生成在喉鳞状细胞癌中的预后相关性。
用LYVE-1作为特异性淋巴管内皮标志物，对78例经组织学诊断为喉癌的患者的临床资料和标本进行染色。测量淋巴管密度（LVD），并分析LVD与肿瘤病例临床病理特征之间的相关性。
喉癌的平均LVD（13.24±5.09）显著高于成人喉乳头状瘤（5.54±3.15）和鳞状上皮发育异常（6.76±4.45，P<0.05）。低分化肿瘤的LVD（15.74±5.24）显著高于中分化肿瘤（13.84±6.20），中分化肿瘤的LVD显著高于高分化肿瘤（11.68±6.34）。0至II期组的LVD（10.66±5.70）显著低于III至IV期组（17.01±6.35）。有淋巴结转移组的LVD（17.25±7.37）显著高于无淋巴结转移组（8.60±5.23，P<0.05）。LVD与年龄、性别、原发部位和远处转移之间无显著相关性。LVD高于平均值的患者总生存期为33.5个月，LVD低于平均值的患者总生存期为81.6个月（P<0.05）。多因素生存分析显示，临床分期和LVD是喉癌的独立预后因素。
LYVE-1染色组织化学显示淋巴管生成主要发生在肿瘤边缘，淋巴管生成在喉癌的发生、癌症进展和淋巴结转移中起重要作用。LVD可能是喉癌预后不良的独立指标。
