Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
J Dent. 2011 Nov;39(11):771-9. doi: 10.1016/j.jdent.2011.08.009. Epub 2011 Aug 19.
Manufacturers are adding fluoride (F) to calcium-containing chewing gums to further promote enamel remineralization. The aim of this study was to assess the effect of a chewing gum containing phosphoryl oligosaccharides of calcium (POs-Ca) and fluoride on remineralization of enamel subsurface lesions, in a double-blind, randomized controlled in situ trial.
Thirty-six volunteer subjects wore removable buccal appliances with three different insets of bovine enamel with subsurface demineralized lesions. For 14 days the subjects chewed one of the three chewing gums (placebo, POs-Ca, POs-Ca+F), three times a day. After each treatment period, the insets were removed from the appliance, embedded, sectioned, polished and then subjected to laboratory tests; mineral level was determined by transverse microradiography (TMR; n=36), and hydroxyapatite (HAp) crystallites were assessed by synchrotron radiation wide-angle X-ray diffraction (WAXRD; n=13). Data were analysed by t-test or Wilcoxon rank-sum test with Bonferroni corrections at 0.05 significance level.
Chewing POs-Ca and POs-Ca+F gums resulted in 21.9±10.6 and 26.3±9.4 (mean±SD) percentage mineral recovery, which was significantly higher than that of placebo gum (15.0±11.4) (p<0.05). Chewing POs-Ca+F gum resulted in 24.9±5.4 (mean±SD) percentage HAp crystallites recovery, which was significantly higher compared to POs-Ca (16.0±4.1%) or placebo (11.1±4.8%) gums (p<0.05).
Addition of POs-Ca to the chewing gum resulted in significant remineralization of enamel subsurface lesions. Although POs-Ca+F gum was not superior in TMR recovery rate when compared with POs-Ca gum, WAXRD results highlighted the importance of fluoride ion bioavailability in the formation of HAp crystallites in enamel subsurface lesions in situ (NCT01377493).
制造商在含氟（F）的钙咀嚼胶中添加氟化物，以进一步促进牙釉质再矿化。本研究的目的是评估含有磷钙（POs-Ca）和氟化物的咀嚼胶对牙釉质表面下病变再矿化的影响，这是一项双盲、随机对照的原位试验。
36 名志愿者佩戴可移动的颊侧矫治器，矫治器上有三个不同的牛牙釉质小块，表面下有脱矿病变。14 天内，受试者每天咀嚼三种咀嚼胶（安慰剂、POs-Ca、POs-Ca+F）三次。每次治疗后，将小块从矫治器上取下，包埋、切片、抛光，然后进行实验室测试；通过横向显微射线照相术（TMR；n=36）测定矿物质水平，通过同步辐射广角 X 射线衍射（WAXRD；n=13）评估羟磷灰石（HAp）微晶。使用 t 检验或 Wilcoxon 秩和检验，采用 Bonferroni 校正，以 0.05 为显著性水平进行数据分析。
咀嚼 POs-Ca 和 POs-Ca+F 口香糖导致 21.9±10.6%和 26.3±9.4%（平均值±标准差）的矿物质恢复率，明显高于安慰剂口香糖（15.0±11.4%）（p<0.05）。咀嚼 POs-Ca+F 口香糖导致 24.9±5.4%（平均值±标准差）的 HAp 微晶恢复率，明显高于 POs-Ca（16.0±4.1%）或安慰剂（11.1±4.8%）口香糖（p<0.05）。
POs-Ca 的添加使牙釉质表面下病变得到显著再矿化。虽然 POs-Ca+F 口香糖在 TMR 恢复率方面并不优于 POs-Ca 口香糖，但 WAXRD 结果强调了氟离子生物利用度在牙釉质表面下病变原位形成 HAp 微晶中的重要性（NCT01377493）。
