Huang Qiwei, Chen Angela Shuyi, Li Qingxin, Kang Congbao
Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), Singapore 138669, Singapore.
Protein Expr Purif. 2011 Dec;80(2):169-75. doi: 10.1016/j.pep.2011.08.008. Epub 2011 Aug 22.
Dengue virus causes serious diseases affecting people in tropical and sub-tropical regions. The nonstructural (NS) protein 2B is an integral membrane protein and important for the regulation of viral protease NS3, which is significant for virus replication. The NS2B-NS3 complex is an important drug target for treating dengue fever. However, little is known about the structure of NS2B in its entirety. Herein, we describe the expression and purification of this integral membrane protein from cell membrane and inclusion bodies of Escherichia coli cells. The initial nuclear magnetic resonance (NMR) and circular dichroism (CD) results indicate that the purified protein adopts alpha-helical structures in LMPG and TDPC micelles.
登革病毒可引发严重疾病，影响热带和亚热带地区的人群。非结构（NS）蛋白2B是一种整合膜蛋白，对病毒蛋白酶NS3的调节至关重要，而NS3对病毒复制具有重要意义。NS2B-NS3复合物是治疗登革热的重要药物靶点。然而，关于NS2B的整体结构我们知之甚少。在此，我们描述了从大肠杆菌细胞膜和包涵体中表达和纯化这种整合膜蛋白的过程。初步的核磁共振（NMR）和圆二色性（CD）结果表明，纯化后的蛋白在LMPG和TDPC胶束中呈α螺旋结构。
