三聚体 G 蛋白 β1γ2 亚基在与模型膜上的 G 蛋白偶联受体激酶 2（GRK2）形成复合物时会改变取向。
Heterotrimeric G protein beta1gamma2 subunits change orientation upon complex formation with G protein-coupled receptor kinase 2 (GRK2) on a model membrane.
机构信息
Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
出版信息
Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E667-73. doi: 10.1073/pnas.1108236108. Epub 2011 Aug 29.
Abstract
Few experimental techniques can assess the orientation of peripheral membrane proteins in their native environment. Sum Frequency Generation (SFG) vibrational spectroscopy was applied to study the formation of the complex between G protein-coupled receptor (GPCR) kinase 2 (GRK2) and heterotrimeric G protein β(1)γ(2) subunits (Gβγ) at a lipid bilayer, without any exogenous labels. The most likely membrane orientation of the GRK2-Gβγ complex differs from that predicted from the known protein crystal structure, and positions the predicted receptor docking site of GRK2 such that it would more optimally interact with GPCRs. Gβγ also appears to change its orientation after binding to GRK2. The developed methodology is widely applicable for the study of other membrane proteins in situ.
摘要
很少有实验技术可以评估外周膜蛋白在其天然环境中的取向。和频发生（SFG）振动光谱被应用于研究在没有任何外源性标记的情况下，G 蛋白偶联受体激酶 2（GRK2）和异三聚体 G 蛋白β（1）γ（2）亚基（Gβγ）在脂质双层中的复合物的形成。GRK2-Gβγ 复合物的最可能的膜取向与从已知的蛋白质晶体结构预测的不同，并将 GRK2 的预测受体对接位点定位，使得它能够与 GPCR 更优化地相互作用。Gβγ 在与 GRK2 结合后似乎也改变了其取向。所开发的方法广泛适用于原位研究其他膜蛋白。
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