Center of Plastic, Hand and Reconstructive Surgery, Department of Trauma Surgery, University Hospital Regensburg, Regensburg, Germany.
Clin Hemorheol Microcirc. 2011;48(1):75-9. doi: 10.3233/CH-2011-1389.
This study was designed to determine if a) hyperbaric oxygen increases the tissue oxygenation of free flaps and b) verification of this effect is possible by using a recently validated and innovative method for two-dimensional pO₂ measurement (Luminescence lifetime imaging = LLI).
Six patients with a free parascapular flap transplanted to the lower limb received hyperbaric oxygen (HBOT) therapy. The HBOT regimen consisted of treatment over 90 minutes with 100% O₂ (FiO₂ 1.0) at 240 kPa (Marx-Schema). The transcutaneous oxygen partial pressure (ptcO₂) was measured over the entire flap with the use of luminescence lifetime imaging (LLI) before and 30, 60, 120 minutes after treatment. The LLI is based on the oxygen dependent quenching of phosphorescence of the indicator dye platinum (II)-octaethyl-porphyrin implemented in a polystyrene sensor foil.
In all six free flaps we could find a significant increase of tissue oxygen over the entire flap in form of increased R-values as well as subsequently calculated absolute ptcO₂ values over a period of 120 min after hyperbaric therapy. The ptcO₂ values increased significantly from 42.59 ± 1.11 Torr before to 81.14 ± 5.95 Torr after hyperbaric treatment (p < 0.001). Even after 2 hours the ptcO₂ values were significantly higher (83.45 ± 13.80 Torr) compared with values prior to HBOT (p < 0.006).
The findings of this study demonstrated an increase of oxygen supply over the entire flap after hyperbaric oxygen therapy.
本研究旨在确定：a）高压氧是否增加游离皮瓣的组织氧合；b）是否可以通过使用最近验证的二维 pO₂测量创新方法（发光寿命成像= LLI）来验证这种效果。
6 例游离肩胛旁皮瓣移植至下肢的患者接受高压氧（HBOT）治疗。HBOT 方案包括在 240 kPa（Marx 方案）下用 100% O₂（FiO₂ 1.0）治疗 90 分钟。在治疗前、治疗后 30、60 和 120 分钟，使用发光寿命成像（LLI）测量整个皮瓣的经皮氧分压（ptcO₂）。LLI 基于氧依赖性猝灭指示剂染料铂（II）-辛基-卟啉的磷光，该磷光在聚苯乙烯传感器箔中实现。
在所有 6 例游离皮瓣中，我们都可以发现整个皮瓣的组织氧显著增加，表现为 R 值增加，以及随后在高压治疗后 120 分钟内计算出的绝对 ptcO₂值增加。ptcO₂ 值从高压治疗前的 42.59±1.11 Torr 显著增加到高压治疗后的 81.14±5.95 Torr（p<0.001）。即使在 2 小时后，ptcO₂ 值仍明显高于高压氧治疗前的值（83.45±13.80 Torr）（p<0.006）。
本研究结果表明，高压氧治疗后整个皮瓣的氧供应增加。
