Landahl Eric C, Antipova Olga, Bongaarts Angela, Barrea Raul, Berry Robert, Binder Lester I, Irving Thomas, Orgel Joseph, Vana Laurel, Rice Sarah E
DePaul University, Department of Physics, 2219 N. Kenmore Ave., Chicago, IL 60614.
Nucl Instrum Methods Phys Res A. 2011 Sep 1;649(1):184-187. doi: 10.1016/j.nima.2011.01.059.
We describe an instrument to record x-ray diffraction patterns from diseased regions of human brain tissue by combining an in-line visible light fluorescence microscope with an x-ray diffraction microprobe. We use thiazine red fluorescence to specifically label and detect the filamentous tau protein pathology associated with Pick's disease, as several labs have done previously. We demonstrate that thiazine red-enhanced regions within the tissue show periodic structure in x-ray diffraction that is not observed in healthy tissue. One observed periodicity (4.2 Å) is characteristic of cross-beta sheet structure, consistent with previous results from powder diffraction studies performed on purified, dried tau protein.
我们描述了一种通过将在线可见光荧光显微镜与X射线衍射微探针相结合来记录人类脑组织病变区域X射线衍射图谱的仪器。正如之前几个实验室所做的那样，我们使用噻嗪红荧光来特异性标记和检测与匹克氏病相关的丝状tau蛋白病变。我们证明，组织内噻嗪红增强区域在X射线衍射中显示出健康组织中未观察到的周期性结构。观察到的一种周期性（4.2 Å）是交叉β片层结构的特征，这与之前对纯化干燥的tau蛋白进行的粉末衍射研究结果一致。
