Berry R W, Sweet A P, Clark F A, Lagalwar S, Lapin B R, Wang T, Topgi S, Guillozet-Bongaarts A L, Cochran E J, Bigio E H, Binder L I
Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 E. Chicago Avenue, Chicago, IL 60611, USA.
J Neurocytol. 2004 May;33(3):287-95. doi: 10.1023/B:NEUR.0000044190.96426.b9.
Filamentous aggregates of the protein tau are a prominent feature of Alzheimer's disease (AD), progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD). However, the extent to which the molecular structure of the tau in these aggregates is similar or differs between these diseases is unclear. We approached this question by examining these disorders with a panel of antibodies that represent different structural, conformational, and cleavage-specific tau epitopes. Although each of these antibodies reveals AD pathology, they resolved into three classes with respect to PSP and CBD: AD2 and Tau-46.1 stained the most tau pathology in all cases; Tau-1, 2, 5, and 12 exhibited variable reactivity; and Tau-66 and MN423 did not reveal any tau pathology. In addition, hippocampal neurofibrillary tangles in these cases showed a predominantly PSP/CBD-like, rather than AD-like, staining pattern. These results indicate that the patterns of the tau epitopes represented by this panel that reside in the pathological aggregates of PSP and CBD are similar to each other but distinct from that of AD.
蛋白质tau的丝状聚集体是阿尔茨海默病（AD）、进行性核上性麻痹（PSP）和皮质基底节变性（CBD）的一个显著特征。然而，这些疾病中tau聚集体的分子结构在何种程度上相似或不同尚不清楚。我们通过使用一组代表不同结构、构象和切割特异性tau表位的抗体来研究这些疾病，从而探讨这个问题。尽管这些抗体中的每一种都能揭示AD病理学特征，但就PSP和CBD而言，它们可分为三类：AD2和Tau-46.1在所有病例中染色的tau病理学特征最多；Tau-1、2、5和12表现出可变的反应性；而Tau-66和MN423未显示任何tau病理学特征。此外，这些病例中的海马神经原纤维缠结显示出主要为PSP/CBD样而非AD样的染色模式。这些结果表明，该组抗体所代表的tau表位在PSP和CBD病理聚集体中的模式彼此相似，但与AD不同。
