Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Neurochem Res. 2011 Dec;36(12):2391-400. doi: 10.1007/s11064-011-0567-2. Epub 2011 Aug 30.
Transdifferentiated and untransdifferentiated mesenchymal stem cells (MSCs) have shown therapeutic benefits in central nervous system (CNS) injury. However, it is unclear which would be more appropriate for transplantation. To address this question, we transplanted untransdifferentiated human umbilical mesenchymal stem cells (HUMSCs) and transdifferentiated HUMSCs (HUMSC-derived neurospheres, HUMSC-NSs) into a rat model of traumatic brain injury. Cognitive function, cell survival and differentiation, brain tissue morphology and neurotrophin expression were compared between groups. Significant improvements in cognitive function and brain tissue morphology were seen in the HUMSCs group compared with HUMSC-NSs group, which was accompanied by increased neurotrophin expression. Moreover, only few grafted cells survived in both the HUMSCs and HUMSC-NSs groups, with very few of the cells differentiating into neural-like cells. These findings indicate that HUMSCs are more appropriate for transplantation and their therapeutic benefits may be associated with neuroprotection rather than cell replacement.
转分化和未转分化的间充质干细胞 (MSCs) 在中枢神经系统 (CNS) 损伤中显示出治疗益处。然而，尚不清楚哪种更适合移植。为了解决这个问题，我们将未分化的人脐带间充质干细胞 (HUMSCs) 和转分化的 HUMSCs (HUMSC 衍生的神经球，HUMSC-NSs) 移植到创伤性脑损伤大鼠模型中。比较了各组的认知功能、细胞存活和分化、脑组织形态和神经营养因子表达。与 HUMSC-NSs 组相比，HUMSCs 组的认知功能和脑组织形态有显著改善，同时神经营养因子表达增加。此外，HUMSCs 和 HUMSC-NSs 两组中仅有少数移植细胞存活，且很少有细胞分化为类神经细胞。这些发现表明 HUMSCs 更适合移植，其治疗益处可能与神经保护而非细胞替代有关。
