Yang Chang-Ching, Shih Yang-Hsin, Ko Miau-Hwa, Hsu Shao-Yun, Cheng Henrich, Fu Yu-Show
Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, Republic of China.
PLoS One. 2008;3(10):e3336. doi: 10.1371/journal.pone.0003336. Epub 2008 Oct 6.
Human umbilical mesenchymal stem cells (HUMSCs) isolated from Wharton's jelly of the umbilical cord can be easily obtained and processed compared with embryonic or bone marrow stem cells. These cells may be a valuable source in the repair of spinal cord injury.
METHODOLOGY/PRINCIPAL FINDINGS: We examine the effects of HUMSC transplantation after complete spinal cord transection in rats. Approximately 5x10(5) HUMSCs were transplanted into the lesion site. Three groups of rats were implanted with either untreated HUMSCs (referred to as the stem cell group), or HUMSCs treated with neuronal conditioned medium (NCM) for either three days or six days (referred to as NCM-3 and NCM-6 days, respectively). The control group received no HUMSCs in the transected spinal cord. Three weeks after transplantation, significant improvements in locomotion were observed in all the three groups receiving HUMSCs (stem cell, NCM-3 and NCM-6 days groups). This recovery was accompanied by increased numbers of regenerated axons in the corticospinal tract and neurofilament-positive fibers around the lesion site. There were fewer microglia and reactive astrocytes in both the rostral and caudal stumps of the spinal cord in the stem cell group than in the control group. Transplanted HUMSCs survived for 16 weeks and produced large amounts of human neutrophil-activating protein-2, neurotrophin-3, basic fibroblast growth factor, glucocorticoid induced tumor necrosis factor receptor, and vascular endothelial growth factor receptor 3 in the host spinal cord, which may help spinal cord repair.
CONCLUSIONS/SIGNIFICANCE: Transplantation of HUMSCs is beneficial to wound healing after spinal cord injury in rats.
与胚胎干细胞或骨髓干细胞相比，从脐带华通氏胶中分离出的人脐带间充质干细胞（HUMSCs）易于获取和处理。这些细胞可能是脊髓损伤修复的宝贵来源。
方法/主要发现：我们研究了大鼠脊髓完全横断后HUMSC移植的效果。将约5×10⁵个HUMSCs移植到损伤部位。三组大鼠分别植入未处理的HUMSCs（称为干细胞组），或用神经元条件培养基（NCM）处理三天或六天的HUMSCs（分别称为NCM - 3天组和NCM - 6天组）。对照组在横断的脊髓中未接受HUMSCs。移植后三周，在接受HUMSCs的所有三组（干细胞组、NCM - 3天组和NCM - 6天组）中均观察到运动能力有显著改善。这种恢复伴随着皮质脊髓束中再生轴突数量的增加以及损伤部位周围神经丝阳性纤维的增加。干细胞组脊髓头端和尾端残端的小胶质细胞和反应性星形胶质细胞均少于对照组。移植的HUMSCs存活了16周，并在宿主脊髓中产生了大量人嗜中性粒细胞激活蛋白 - 2、神经营养因子 - 3、碱性成纤维细胞生长因子、糖皮质激素诱导的肿瘤坏死因子受体和血管内皮生长因子受体3，这可能有助于脊髓修复。
结论/意义：HUMSCs移植有利于大鼠脊髓损伤后的伤口愈合。
