Steden M, Duntze W
Institut für Physiologische Chemie, Ruhr-Universität Bochum, F.R.G.
FEMS Microbiol Lett. 1990 Mar 15;56(3):229-32. doi: 10.1016/s0378-1097(05)80045-x.
The SSL1 gene of S. cerevisiae which is involved in the cell-type specific recovery of mating type alpha cells from cell cycle arrest by the mating hormone a-factor has been cloned as a 1 kb Sau3A fragment in a complementing plasmid from a library of S. cerevisae genomic DNA. Disruption of this gene in wild-type alpha-cells results in the ssl1- phenotype which is characterized by an extreme sensitivity to a-factor. Transcription analysis revealed that the SSL1 gene is transcribed only in MAT alpha cells but not in MAT alpha or diploid (MAT alpha/MAT alpha) cells defining it as a member of the set of alpha-specific genes.
酿酒酵母的SSL1基因参与α细胞通过交配激素a因子从细胞周期停滞中进行细胞类型特异性恢复，该基因已作为一个1 kb的Sau3A片段，从酿酒酵母基因组DNA文库的互补质粒中克隆出来。在野生型α细胞中破坏该基因会导致ssl1-表型，其特征是对a因子极度敏感。转录分析表明，SSL1基因仅在MATα细胞中转录，而在MATα或二倍体（MATα/MATα）细胞中不转录，这将其定义为一组α特异性基因的成员。
