Hisatomi T, Tsuboi M
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Japan.
Curr Genet. 1989 Jun;15(6):399-401. doi: 10.1007/BF00376794.
The DNA of Saccharomyces exiguus was analyzed by Southern hybridization using cloned MATa, MAT alpha, and HO genes of Saccharomyces cerevisiae as probes. It was shown that S. exiguus has a DNA sequence homologous with the HO gene of S. cerevisiae and that this DNA sequence is on a chromosome of about 940 kb of DNA in S. exiguus. However, there is no DNA sequence in S. exiguus that is homologous with the MAT genes of S. cerevisiae.
使用酿酒酵母的克隆MATa、MATα和HO基因作为探针,通过Southern杂交分析了嗜杀酵母的DNA。结果表明,嗜杀酵母具有与酿酒酵母HO基因同源的DNA序列,并且该DNA序列位于嗜杀酵母中约940 kb DNA的一条染色体上。然而,嗜杀酵母中没有与酿酒酵母MAT基因同源的DNA序列。