Goggin Melissa M, Gozum Stephanie D, Burrows David M, Nguyen An, Corcoran Henry F, Dotzauer Heather, Tann Cheng-Min, Lundberg Richard A, Janis Gregory C
Research & Development Laboratory, MEDTOX Laboratories, New Brighton, MN 55112, USA.
Bioanalysis. 2011 Aug;3(16):1837-46. doi: 10.4155/bio.11.180.
Patients with iron-deficiency anemia benefit from intravenous iron therapies. Development of these pharmaceutical agents requires pharmacokinetic studies monitoring levels of both the administered agent and transferrin-bound iron (TBI). Successful pharmacokinetic methods must discriminate iron species.
Routine colorimetric procedures were used to reliably measure total iron and TBI following iron-sucrose administration. Iron was liberated from iron-sucrose allowing the determination of all circulating iron. Solid-phase sample processing allowed the measurement of TBI. Circulating iron-sucrose could then be calculated as the difference between total iron and TBI.
A reproducible and robust spectrophotometric method was developed and validated for measuring total iron and TBI in human serum following iron-sucrose therapy.
缺铁性贫血患者可从静脉铁剂治疗中获益。这些药物制剂的研发需要进行药代动力学研究，监测所给药剂和转铁蛋白结合铁（TBI）的水平。成功的药代动力学方法必须能够区分铁的种类。
采用常规比色法可靠地测定了蔗糖铁给药后的总铁和TBI。铁从蔗糖铁中释放出来，从而能够测定所有循环铁。固相样品处理可用于测定TBI。然后可将循环蔗糖铁计算为总铁与TBI之间的差值。
开发并验证了一种可重复且稳健的分光光度法，用于测定蔗糖铁治疗后人血清中的总铁和TBI。
