用于蔗糖铁制剂药代动力学研究的人血清中总铁和转铁蛋白结合铁的分析。
Analysis of total and transferrin-bound iron in human serum for pharmacokinetic studies of iron-sucrose formulations.
作者信息
Goggin Melissa M, Gozum Stephanie D, Burrows David M, Nguyen An, Corcoran Henry F, Dotzauer Heather, Tann Cheng-Min, Lundberg Richard A, Janis Gregory C
机构信息
Research & Development Laboratory, MEDTOX Laboratories, New Brighton, MN 55112, USA.
出版信息
Bioanalysis. 2011 Aug;3(16):1837-46. doi: 10.4155/bio.11.180.
BACKGROUND
Patients with iron-deficiency anemia benefit from intravenous iron therapies. Development of these pharmaceutical agents requires pharmacokinetic studies monitoring levels of both the administered agent and transferrin-bound iron (TBI). Successful pharmacokinetic methods must discriminate iron species.
RESULTS
Routine colorimetric procedures were used to reliably measure total iron and TBI following iron-sucrose administration. Iron was liberated from iron-sucrose allowing the determination of all circulating iron. Solid-phase sample processing allowed the measurement of TBI. Circulating iron-sucrose could then be calculated as the difference between total iron and TBI.
CONCLUSION
A reproducible and robust spectrophotometric method was developed and validated for measuring total iron and TBI in human serum following iron-sucrose therapy.
背景
缺铁性贫血患者可从静脉铁剂治疗中获益。这些药物制剂的研发需要进行药代动力学研究，监测所给药剂和转铁蛋白结合铁（TBI）的水平。成功的药代动力学方法必须能够区分铁的种类。
结果
采用常规比色法可靠地测定了蔗糖铁给药后的总铁和TBI。铁从蔗糖铁中释放出来，从而能够测定所有循环铁。固相样品处理可用于测定TBI。然后可将循环蔗糖铁计算为总铁与TBI之间的差值。
结论
开发并验证了一种可重复且稳健的分光光度法，用于测定蔗糖铁治疗后人血清中的总铁和TBI。
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