[年龄与精子DNA碎片化的关联]
[Association of age with sperm DNA fragmentation].
作者信息
Horta Fabrizzio, Madariaga Marcia, García Alejandra, Hartel Steffen, Smith Rosita
机构信息
Instituto de Investigaciones Materno Infantil, Universidad de Chile, Santiago, Chile.
出版信息
Rev Med Chil. 2011 Mar;139(3):306-12. Epub 2011 Aug 25.
BACKGROUND
There is an association between aging ana an increased number of sperms with alterations in nuclear DNA.
AIM
To study the association between age and fragmentation of sperm DNA.
MATERIAL AND METHODS
Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersion test) assays.
RESULTS
Compared with their younger counterparts, patients aged more than 40 years had a higher proportion of sperms with DNA fragmentation by TUNEL (20 ± 1.3 and 24 ± 1.9% respectively, p < 0.05) and SCD (22 ± 1.4 and 26 ± 1.6% respectively, p < 0.05). The results of both assays had a correlation coefficient of O.8. No differences between groups were observed for other seminal parameters.
CONCLUSIONS
Sperm DNA fragmentation increases with age in males.
背景
衰老与精子数量增加以及核DNA改变之间存在关联。
目的
研究年龄与精子DNA碎片化之间的关联。
材料与方法
62名志愿者提供精液用于分析。他们被分为年龄小于40岁的一组和年龄大于40岁的另一组。通过TUNEL（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记）和SCD（精子染色质扩散试验）检测来研究精子DNA碎片化。
结果
与年轻男性相比，年龄大于40岁的患者通过TUNEL检测显示DNA碎片化的精子比例更高（分别为20±1.3%和24±1.9%，p<0.05），通过SCD检测也更高（分别为22±1.4%和26±1.6%，p<0.05）。两种检测结果的相关系数为0.8。两组在其他精液参数方面未观察到差异。
结论
男性精子DNA碎片化随年龄增加。
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