New Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Rutgers University , 59 Dudley Road, New Brunswick, New Jersey 08901, United States.
J Agric Food Chem. 2011 Oct 12;59(19):10586-93. doi: 10.1021/jf202438d. Epub 2011 Sep 13.
A rapid and comprehensive qualitative method has been developed to characterize the different classes of polyphenols, such as anthocyanins, flavonols, phenolic acids, and flavanols/proanthocyanidins, in grape products. The detection was achieved by two runs with the same LC gradient in different MS ionization modes and mobile phase modifiers (positive ionization mode and 0.4% trifluoroacetic acid for anthocyanins and flavonols; negative ionization mode and 0.1% formic acid for phenolic acids and flavanols). From an analysis of the MS and UV data and in comparison with the authenticated standards, a total of 53 compounds were identified, including 33 anthocyanins, 12 flavonols, 4 phenolic acids, and 4 flavanols/proanthocyanidins. With the method developed, a survey was then conducted to qualitatively assess the composition of polyphenols among 29 different grape products including original grape, grape juice, grape wine, and grape-derived dietary supplements, and their chemical profiles were systematically compared. This method provided a comprehensive qualitative insight into the composition of polyphenols in grape-derived products.
已经开发出一种快速而全面的定性方法，用于鉴定葡萄产品中不同类别的多酚，如花色苷、类黄酮、酚酸和黄烷醇/原花青素。通过在不同的 MS 电离模式和流动相修饰剂（正离子模式和 0.4%三氟乙酸用于花色苷和类黄酮；负离子模式和 0.1%甲酸用于酚酸和黄烷醇）下进行两次相同 LC 梯度的运行来实现检测。通过对 MS 和 UV 数据的分析，并与经过验证的标准进行比较，共鉴定出 53 种化合物，包括 33 种花色苷、12 种类黄酮、4 种酚酸和 4 种黄烷醇/原花青素。使用所开发的方法，对 29 种不同的葡萄产品（包括原葡萄、葡萄汁、葡萄酒和葡萄衍生的膳食补充剂）中的多酚组成进行了定性评估，并对其化学特征进行了系统比较。该方法提供了对葡萄衍生产品中多酚组成的全面定性见解。
