分析人副流感病毒 3 型基因组反义启动子 13-96 个核苷酸序列揭示了正、负调控复制元件。
Analysis of nucleotides 13-96 of the human parainfluenza virus type 3 antigenomic promoter reveals positive- and negative-acting replication elements.
机构信息
Department of Microbiology, University of Wisconsin-La Crosse, La Crosse, WI 54601, USA.
出版信息
Virology. 2011 Oct 25;419(2):90-6. doi: 10.1016/j.virol.2011.08.003. Epub 2011 Aug 30.
During replication of human parainfluenza virus type 3 (HPIV3), the 96-nucleotide antigenomic promoter (AGP) of HPIV3 directs the synthesis of genomic RNA. Previous work showed that nucleotides 1-12 were critical in promoting replication of an HPIV3 minireplicon, but nucleotides 13-96 were not investigated. In this study, the role of nucleotides 13-96 in AGP function was analyzed by creating and assaying mutations in an HPIV3 minireplicon. A replication promoting element known as promoter element II (nt 79-96) was confirmed in the HPIV3 AGP. Additionally, nucleotides 13-39 were found to constitute an additional positive-acting cis-element. However, detailed analysis of the 13-39 element revealed a complicated control element with both stimulatory and repressing elements. Specifically, nucleotides 21-28 were shown to repress RNA replication, while flanking sequences had a stimulatory effect.
在人类副流感病毒 3 型（HPIV3）的复制过程中，HPIV3 的 96 个核苷酸的抗原基因组启动子（AGP）指导基因组 RNA 的合成。之前的工作表明，核苷酸 1-12 对促进 HPIV3 微小复制子的复制至关重要，但核苷酸 13-96 并未进行研究。在这项研究中，通过在 HPIV3 微小复制子中创建和分析突变，分析了核苷酸 13-96 在 AGP 功能中的作用。在 HPIV3 AGP 中证实了一个已知的复制促进元件，即启动子元件 II（nt 79-96）。此外，还发现核苷酸 13-39 构成了另一个具有正向作用的顺式作用元件。然而，对 13-39 元件的详细分析揭示了一个具有刺激和抑制元件的复杂调控元件。具体来说，核苷酸 21-28 被证明抑制 RNA 复制，而侧翼序列则具有刺激作用。
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