State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Bioresour Technol. 2011 Oct;102(20):9705-9. doi: 10.1016/j.biortech.2011.08.024. Epub 2011 Aug 10.
Corn stover (CS) hydrolysate was used as the fermentation feedstock of Trichosporon cutaneum CX1 for production of microbial lipid as the potential raw material of biodiesel. Two major technical barriers of the lipid fermentation were investigated: one was the strong inhibition of lignocellulose degradation compounds generated in the CS pretreatment; the other was the low carbon-to-nitrogen molar ratio (C/N ratio) of the CS hydrolysate. The newly established biodetoxification method was applied to remove the inhibitors in the pretreated CS. The enhancement of the pretreatment severity and the biodetoxification intensity on the lipid fermentation was investigated. The results show that the biodetoxification not only efficiently removed the inhibitor substances, but also led to the reduction of nitrogen content and the increase of C/N ratio. The cell lipid content of T. cutaneum CX1 using the biodetoxified CS hydrolysate reached 23.5%, which was doubled than that using the non-detoxified value.
玉米秸秆（CS）水解物被用作产朊假丝酵母 CX1 的发酵原料，以生产微生物油脂作为生物柴油的潜在原料。研究了脂质发酵的两个主要技术障碍：一是 CS 预处理中产生的木质纤维素降解化合物的强烈抑制作用；另一个是 CS 水解物的碳氮摩尔比（C/N 比）低。新建立的生物解毒方法用于去除预处理 CS 中的抑制剂。研究了增强预处理的严格程度和生物解毒强度对脂质发酵的影响。结果表明，生物解毒不仅有效地去除了抑制剂物质，而且还导致氮含量降低和 C/N 比增加。使用生物解毒的 CS 水解物的产朊假丝酵母 CX1 的细胞脂质含量达到 23.5％，是未解毒值的两倍。
