Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
Bioresour Technol. 2013 Sep;143:18-24. doi: 10.1016/j.biortech.2013.05.102. Epub 2013 May 30.
The effect of medium composition and cultural condition on the growth and lipid accumulation of oleaginous yeast Trichosporon cutaneum on corncob acid hydrolysate was systematically investigated. Glucose, xylose, and cellobiose were shown to be promising sugar for lipid production by T. cutaneum. Adding other nitrogen sources into the hydrolysate was not beneficial for the lipid production possibly due to the existence of other nitrogen sources in it. Interestingly, adding MgSO4·7H2O, CuSO4·5H2O, MnSO4·H2O, and KCl (optimal concentration were 0.3, 3.0×10(-3), 3.0×10(-3), and 0.4 g/L, respectively) could stimulate the lipid production by T. cutaneum. Additionally, inoculum concentration, temperature, and initial pH (optimal value were 5%, 28 °C, and 6.0, respectively) showed influence on the lipid production of T. cutaneum. Under the optimum conditions, the biomass (22.9 g/L) had a weak increase (3.6%), while the lipid content (45.4%) and lipid coefficient (22.9%) increased obviously (about 26.5% and 31.6%) compared with the initial conditions.
系统研究了培养基组成和培养条件对脂肪酵母瘤胃假丝酵母在玉米芯酸水解物上生长和脂类积累的影响。结果表明，葡萄糖、木糖和纤维二糖是瘤胃假丝酵母生产油脂的理想糖。向水解物中添加其他氮源对油脂生产没有好处，可能是因为水解物中存在其他氮源。有趣的是，添加 MgSO4·7H2O、CuSO4·5H2O、MnSO4·H2O 和 KCl（最佳浓度分别为 0.3、3.0×10(-3)、3.0×10(-3)和 0.4 g/L）可以刺激瘤胃假丝酵母的脂类生产。此外，接种浓度、温度和初始 pH 值（最佳值分别为 5%、28°C 和 6.0）对瘤胃假丝酵母的脂类生产也有影响。在最佳条件下，生物量（22.9 g/L）仅略有增加（3.6%），而脂含量（45.4%）和脂系数（22.9%）明显增加（约 26.5%和 31.6%）。
