Marley Richard, Baines Richard A
Cold Spring Harb Protoc. 2011 Sep 1;2011(9):pdb.prot065649. doi: 10.1101/pdb.prot065649.
The fruit fly Drosophila melanogaster has been instrumental in expanding our understanding of early aspects of neural development. The use of this model system has greatly added to our knowledge of neural cell-fate determination, axon guidance, and synapse formation. It has also become possible to access and make electrophysiological recordings directly from neurons in situ in an intact central nervous system (CNS), which has facilitated studies of the development and regulation of neuronal signaling. It is possible to obtain electrophysiological recordings from all stages of Drosophila. Exposure of the intact Drosophila CNS is a prerequisite for such electrophysiological recordings. The dissection procedure described here can be applied to both late-stage embryos (stage 16 onward) and larvae. Because of their size, third-instar larvae are more difficult to flatten using this method and, if recording from this stage, the reader might consider using insect pins for the dissection or isolating the CNS using an alternative method. The dissection should take <10 min if all preparation work has been completed in advance. Owing to the short life span of the dissected larva, it is not recommended that the procedure be stopped or the preparation stored for later use.
果蝇黑腹果蝇在拓展我们对神经发育早期方面的理解上发挥了重要作用。使用这个模型系统极大地增加了我们对神经细胞命运决定、轴突导向和突触形成的认识。还能够直接在完整的中枢神经系统（CNS）中原位从神经元获取并进行电生理记录，这促进了对神经元信号传导的发育和调节的研究。从果蝇的各个阶段都有可能获得电生理记录。完整果蝇中枢神经系统的暴露是进行此类电生理记录的前提条件。这里描述的解剖程序可应用于晚期胚胎（16期及以后）和幼虫。由于其大小，三龄幼虫使用这种方法更难展平，如果从这个阶段进行记录，读者可以考虑使用昆虫针进行解剖或使用替代方法分离中枢神经系统。如果所有准备工作提前完成，解剖应在10分钟内完成。由于解剖后的幼虫寿命较短，不建议停止该程序或保存标本以供以后使用。
