Marley Richard, Baines Richard A
Cold Spring Harb Protoc. 2011 Sep 1;2011(9):pdb.prot065664. doi: 10.1101/pdb.prot065664.
The fruit fly Drosophila melanogaster has been instrumental in expanding our understanding of early aspects of neural development. The use of this model system has greatly added to our knowledge of neural cell-fate determination, axon guidance, and synapse formation. It has also become possible to access and make electrophysiological recordings directly from neurons in situ in an intact central nervous system (CNS), which has facilitated studies of the development and regulation of neuronal signaling. This protocol describes a procedure for revealing larval motor neurons and applying whole-cell patch recording techniques to these cells. The useful lifetime of first-instar larval preparations is ∼30 min, and that of third-instar CNS preparations is up to 1 h. It is therefore recommended that fresh preparations are used and that no breaks are taken during the procedure, although there may be time to pull and polish a patch pipette.
果蝇黑腹果蝇在拓展我们对神经发育早期方面的理解上发挥了重要作用。这个模型系统的使用极大地增进了我们对神经细胞命运决定、轴突导向和突触形成的认识。直接从完整中枢神经系统（CNS）原位的神经元进行电生理记录也已成为可能，这推动了对神经元信号传导的发育和调节的研究。本方案描述了一种揭示幼虫运动神经元并将全细胞膜片钳记录技术应用于这些细胞的程序。一龄幼虫标本的有效使用时长约为30分钟，三龄中枢神经系统标本的有效使用时长可达1小时。因此，建议使用新鲜标本，并且在操作过程中不要中断，不过可能有时间拉制和打磨膜片钳电极。
