Department of Animal Science, University of Vermont, Burlington, VT, USA.
J Nutr. 2011 Oct;141(10):1819-26. doi: 10.3945/jn.111.143776. Epub 2011 Aug 31.
Trans-fatty acid consumption from partially hydrogenated vegetable oil (PHVO) has been positively associated with multiple cardiovascular disease risk factors and events. This study was designed to examine the effects of trans-fatty acid isomer profile of PHVO on plasma lipids and lipoproteins and hepatic expression of key genes involved in cholesterol and fatty acid metabolism. Thirty-three male F(1)B strain Syrian Golden Hamsters were allocated to 1 of 3 hypercholesterolemic diets containing (5% by weight): 1) tristearin [control fat (CON)]; 2) partially hydrogenated high-oleic acid sunflower oil (PH-SUN); or 3) partially hydrogenated high-linoleic acid safflower oil (PH-SAF). PH-SUN contained more trans-4 to trans-10 18:1 compared with PH-SAF, which contained more trans-11 to trans-16 18:1. The addition of both PHVO to the diet increased plasma total cholesterol concentrations relative to CON, but only PH-SUN increased the plasma ratio of non-HDL:HDL cholesterol compared with CON. PH-SUN increased VLDL (total, large, and medium) and IDL particle concentrations while decreasing total, medium, and small HDL particle concentrations relative to CON. Both PHVO diets increased the hepatic cholesterol ester concentration, whereas the hepatic TG concentration was lower in PH-SUN compared with PH-SAF and CON. Levels of hepatic LDL receptor, HMG-CoA reductase, and sterol response element binding protein 1 mRNA were specifically reduced in the PH-SUN group compared to the CON group. Expression of SREBP1c was upregulated in both PHVO groups compared to CON, whereas only the PH-SAF group had higher levels of the lipogenic enzymes acetyl-CoA carboxylase, fatty acid synthase, and stearoyl-CoA desaturase-1 compared to CON. These results indicate that differences in the trans-fatty acid profile of PHVO can differentially affect lipid and lipoprotein metabolism.
反式脂肪酸（trans-fatty acid）来源于部分氢化植物油（partially hydrogenated vegetable oil，PHVO），它与多种心血管疾病的风险因素和事件呈正相关。本研究旨在探究 PHVO 中反式脂肪酸异构体组成对血浆脂质和脂蛋白以及胆固醇和脂肪酸代谢关键基因在肝脏中表达的影响。33 只雄性 F(1)B 品系叙利亚金仓鼠被分为 3 组，每组接受 1 种高胆固醇饮食，分别含有（按重量计）：1）三硬脂酸甘油酯（对照脂肪，CON）；2）部分氢化高油酸葵花籽油（PH-SUN）；或 3）部分氢化高亚油酸红花籽油（PH-SAF）。与 PH-SAF 相比，PH-SUN 中含有更多的反式 4-反式 10 18:1，而 PH-SAF 中含有更多的反式 11-反式 16 18:1。与 CON 相比，添加 PHVO 会增加血浆总胆固醇浓度，但只有 PH-SUN 会增加非高密度脂蛋白胆固醇与高密度脂蛋白胆固醇的比值。与 CON 相比，PH-SUN 增加了 VLDL（总、大、中）和 IDL 颗粒浓度，同时降低了总、中、小 HDL 颗粒浓度。两种 PHVO 饮食均增加了肝脏胆固醇酯浓度，而 PH-SUN 组的肝脏三酰甘油浓度低于 PH-SAF 组和 CON 组。与 CON 组相比，PH-SUN 组的 LDL 受体、HMG-CoA 还原酶和固醇反应元件结合蛋白 1 mRNA 水平特异性降低。与 CON 组相比，两种 PHVO 组的 SREBP1c 表达均上调，而只有 PH-SAF 组的乙酰辅酶 A 羧化酶、脂肪酸合酶和硬脂酰辅酶 A 去饱和酶-1 的脂肪生成酶水平高于 CON 组。这些结果表明，PHVO 中反式脂肪酸组成的差异会对脂质和脂蛋白代谢产生不同的影响。
