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来自粟酒裂殖酵母的U1小核RNA具有独特且保守的特征，由一个必需的单拷贝基因编码。

U1 small nuclear RNA from Schizosaccharomyces pombe has unique and conserved features and is encoded by an essential single-copy gene.

作者信息

Porter G, Brennwald P, Wise J A

机构信息

Department of Biochemistry, University of Illinois, Urbana 61801.

出版信息

Mol Cell Biol. 1990 Jun;10(6):2874-81. doi: 10.1128/mcb.10.6.2874-2881.1990.

DOI:10.1128/mcb.10.6.2874-2881.1990
PMID:2188102
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360649/
Abstract

We have cloned, sequenced, and disrupted the gene encoding U1 small nuclear RNA (snRNA) in the fission yeast Schizosaccharomyces pombe. This RNA is close in size and exhibits a high degree of secondary structure homology to human U1 RNA. There exist two regions of extended primary sequence identity between S. pombe and human U1 RNAs; the first comprises nucleotides involved in hydrogen bonding to 5' splice junctions, and the second is a single-stranded region which, in the human snRNA, forms part of the A protein binding site. S. pombe U1 lacks two nucleotides just following the 5' cap structure which are present in all other U1 homologs examined to date, and the region which corresponds to the binding site for the human 70K protein (molecular weight of 55,000) is more divergent than in other organisms. A putative upstream transcription signal is conserved in sequence and location among all loci encoding spliceosomal snRNAs in S. pombe with the exception of U6. Disruption of the single-copy U1 gene, designated snu1, reveals that this RNA is indispensable for viability.

摘要

我们已经克隆、测序并破坏了裂殖酵母粟酒裂殖酵母中编码U1小核RNA（snRNA）的基因。这种RNA在大小上相近，并且与人类U1 RNA在二级结构上具有高度同源性。粟酒裂殖酵母和人类U1 RNA之间存在两个延伸的一级序列相同区域；第一个区域包含与5'剪接接头形成氢键的核苷酸，第二个区域是一个单链区域，在人类snRNA中，它构成A蛋白结合位点的一部分。粟酒裂殖酵母U1在5'帽结构之后缺少两个核苷酸，而这两个核苷酸在迄今为止检测的所有其他U1同源物中都存在，并且与人类70K蛋白（分子量为55,000）结合位点相对应的区域比其他生物体中的差异更大。除U6外，在粟酒裂殖酵母中所有编码剪接体snRNA的基因座中，一个推定的上游转录信号在序列和位置上都是保守的。破坏单拷贝的U1基因（命名为snu1）表明，这种RNA对于细胞存活是必不可少的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b0e/360649/8ad2efa7e75d/molcellb00042-0448-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b0e/360649/da047403650e/molcellb00042-0446-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b0e/360649/8ad2efa7e75d/molcellb00042-0448-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b0e/360649/da047403650e/molcellb00042-0446-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b0e/360649/8ad2efa7e75d/molcellb00042-0448-a.jpg

相似文献

1
U1 small nuclear RNA from Schizosaccharomyces pombe has unique and conserved features and is encoded by an essential single-copy gene.来自粟酒裂殖酵母的U1小核RNA具有独特且保守的特征，由一个必需的单拷贝基因编码。
Mol Cell Biol. 1990 Jun;10(6):2874-81. doi: 10.1128/mcb.10.6.2874-2881.1990.
2
Cloning of Schizosaccharomyces pombe genes encoding the U1, U2, U3 and U4 snRNAs.粟酒裂殖酵母中编码U1、U2、U3和U4小核RNA的基因克隆
Gene. 1989 Sep 30;81(2):227-35. doi: 10.1016/0378-1119(89)90183-2.
3
U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals.U2小核RNA在粟酒裂殖酵母和哺乳动物之间具有显著的保守性。
Mol Cell Biol. 1988 Dec;8(12):5575-80. doi: 10.1128/mcb.8.12.5575-5580.1988.
4
The two similarly expressed genes encoding U3 snRNA in Schizosaccharomyces pombe lack introns.裂殖酵母中两个编码U3小核仁RNA的相似表达基因不含内含子。
Mol Biol Evol. 1992 Mar;9(2):297-308. doi: 10.1093/oxfordjournals.molbev.a040721.
5
The sequence of U3 from Schizosaccharomyces pombe suggests structural divergence of this snRNA between metazoans and unicellular eukaryotes.来自粟酒裂殖酵母的U3序列表明，后生动物和单细胞真核生物之间这种小核仁RNA存在结构差异。
Nucleic Acids Res. 1988 Nov 11;16(21):10131-52. doi: 10.1093/nar/16.21.10131.
6
Schizosaccharomyces pombe U4 small nuclear RNA closely resembles vertebrate U4 and is required for growth.粟酒裂殖酵母U4小核RNA与脊椎动物U4非常相似，是生长所必需的。
J Mol Biol. 1989 Aug 5;208(3):371-9. doi: 10.1016/0022-2836(89)90502-0.
7
A mutation in a single gene of Schizosaccharomyces pombe affects the expression of several snRNAs and causes defects in RNA processing.粟酒裂殖酵母单个基因的突变会影响几种小核RNA的表达，并导致RNA加工缺陷。
EMBO J. 1990 Feb;9(2):525-34. doi: 10.1002/j.1460-2075.1990.tb08139.x.
8
Saccharomyces cerevisiae U1 small nuclear RNA secondary structure contains both universal and yeast-specific domains.酿酒酵母U1小核RNA二级结构包含通用结构域和酵母特异性结构域。
Proc Natl Acad Sci U S A. 1990 Jan;87(2):851-5. doi: 10.1073/pnas.87.2.851.
9
Mutational analysis of U1 function in Schizosaccharomyces pombe: pre-mRNAs differ in the extent and nature of their requirements for this snRNA in vivo.粟酒裂殖酵母中U1功能的突变分析：前体mRNA在体内对这种小核RNA的需求程度和性质存在差异。
RNA. 1996 May;2(5):404-18.
10
The gene for the U6 small nuclear RNA in fission yeast has an intron.裂殖酵母中U6小核RNA的基因含有一个内含子。
Nature. 1989 Jan 5;337(6202):87-90. doi: 10.1038/337087a0.

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The conserved AU dinucleotide at the 5' end of nascent U1 snRNA is optimized for the interaction with nuclear cap-binding-complex.新生U1小核RNA 5'端保守的AU二核苷酸经过优化，以利于与核帽结合复合物相互作用。
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本文引用的文献

1
Plasmids carrying the yeast OMP decarboxylase structural and regulatory genes: transcription regulation in a foreign environment.携带酵母OMP脱羧酶结构基因和调控基因的质粒：在异源环境中的转录调控
Cell. 1983 Feb;32(2):371-7. doi: 10.1016/0092-8674(83)90456-7.
2
Evolutionary divergence of the mRNA transcription initiation mechanism in yeast.酵母中mRNA转录起始机制的进化分歧
Nature. 1983 Jan 13;301(5896):167-9. doi: 10.1038/301167a0.
3
U2 RNA shares a structural domain with U1, U4, and U5 RNAs.U2 RNA与U1、U4和U5 RNA共有一个结构域。
Prp4激酶赋予剪接许可：剪接体激活过程中对弱剪接位点的控制。
PLoS Genet. 2016 Jan 5;12(1):e1005768. doi: 10.1371/journal.pgen.1005768. eCollection 2016 Jan.
4
The Sm complex is required for the processing of non-coding RNAs by the exosome.Sm 复合物是 exosome 加工非编码 RNA 所必需的。
PLoS One. 2013 Jun 6;8(6):e65606. doi: 10.1371/journal.pone.0065606. Print 2013.
5
Proteomic analysis of the U1 snRNP of Schizosaccharomyces pombe reveals three essential organism-specific proteins.粟酒裂殖酵母U1 snRNP的蛋白质组分析揭示了三种必需的物种特异性蛋白质。
Nucleic Acids Res. 2007;35(5):1391-401. doi: 10.1093/nar/gkl1144. Epub 2007 Jan 30.
6
The splicing factor U2AF small subunit is functionally conserved between fission yeast and humans.剪接因子U2AF小亚基在裂殖酵母和人类之间功能保守。
Mol Cell Biol. 2004 May;24(10):4229-40. doi: 10.1128/MCB.24.10.4229-4240.2004.
7
Pre-mRNA splicing in Schizosaccharomyces pombe: regulatory role of a kinase conserved from fission yeast to mammals.粟酒裂殖酵母中的前体mRNA剪接：一种从裂殖酵母到哺乳动物保守的激酶的调控作用
Curr Genet. 2003 Feb;42(5):241-51. doi: 10.1007/s00294-002-0355-2. Epub 2002 Dec 13.
8
Transcription of the Schizosaccharomyces pombe U2 gene in vivo and in vitro is directed by two essential promoter elements.粟酒裂殖酵母U2基因在体内和体外的转录由两个必需的启动子元件指导。
Nucleic Acids Res. 2001 May 15;29(10):2003-11. doi: 10.1093/nar/29.10.2003.
9
Pac1p, an RNase III homolog, is required for formation of the 3' end of U2 snRNA in Schizosaccharomyces pombe.Pac1p是一种核糖核酸酶III同源物，在粟酒裂殖酵母中，它是U2小核RNA 3'末端形成所必需的。
RNA. 1999 Aug;5(8):1083-98. doi: 10.1017/s1355838299990726.
10
Molecular genetic analysis of U2AF59 in Schizosaccharomyces pombe: differential sensitivity of introns to mutational inactivation.粟酒裂殖酵母中U2AF59的分子遗传学分析：内含子对突变失活的差异敏感性
RNA. 1999 Jan;5(1):49-65. doi: 10.1017/s1355838299981323.
EMBO J. 1982;1(10):1259-65. doi: 10.1002/j.1460-2075.1982.tb00022.x.
4
The conformation of chicken, rat and human U1A RNAs in solution.溶液中鸡、大鼠和人类U1A核糖核酸的构象。
Nucleic Acids Res. 1981 Feb 25;9(4):841-58. doi: 10.1093/nar/9.4.841.
5
A compensatory base change in U1 snRNA suppresses a 5' splice site mutation.U1小核核糖核酸中的一种补偿性碱基变化抑制了5'剪接位点突变。
Cell. 1986 Sep 12;46(6):827-35. doi: 10.1016/0092-8674(86)90064-4.
6
An essential yeast snRNA with a U5-like domain is required for splicing in vivo.一种具有类似U5结构域的必需酵母小核RNA在体内剪接过程中是必需的。
Cell. 1987 Jun 5;49(5):613-24. doi: 10.1016/0092-8674(87)90537-x.
7
Molecular characterisation of the DNA ligase gene, CDC17, from the fission yeast Schizosaccharomyces pombe.来自裂殖酵母粟酒裂殖酵母的DNA连接酶基因CDC17的分子特征分析。
Eur J Biochem. 1987 Feb 2;162(3):659-67. doi: 10.1111/j.1432-1033.1987.tb10688.x.
8
U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs.酵母中的U2 RNA出人意料地大，并且与脊椎动物的U4、U5和U6小核RNA具有同源性。
Cell. 1986 Oct 10;47(1):49-59. doi: 10.1016/0092-8674(86)90365-x.
9
Schizosaccharomyces pombe and Saccharomyces cerevisiae: a look at yeasts divided.粟酒裂殖酵母和酿酒酵母：剖析分裂的酵母
Cell. 1986 Jun 20;45(6):781-2. doi: 10.1016/0092-8674(86)90550-7.
10
Plant small nuclear RNAs. IV. The structure of U1 RNA from Chlorella saccharophila: a phylogenetic support, in terms of RNA structure, for the probable interaction between U1 and U2 and RNPs during the splicing of pre-mRNA.植物小核RNA。IV。嗜糖小球藻U1 RNA的结构：就RNA结构而言，为前体mRNA剪接过程中U1与U2及核糖核蛋白颗粒之间可能的相互作用提供了系统发育学支持。
Nucleic Acids Res. 1988 Mar 25;16(6):2734. doi: 10.1093/nar/16.6.2734.