Dandekar T, Tollervey D
Genie Microbiologique, Institut Pasteur, Paris, France.
Gene. 1989 Sep 30;81(2):227-35. doi: 10.1016/0378-1119(89)90183-2.
Schizosaccharomyces pombe contains a group of five relatively abundant small nuclear RNAs (snRNAs) which are immunoprecipitated by human autoimmune antibodies of Sm serotype. The S. pombe RNAs hybridise to probes specific for human U1, U2, U4, U5 and U6 and in each case are similar in size to the human species. A further group of snRNAs from S. pombe are precipitated by antibodies against U3 containing ribonucleoprotein; the most abundant of these species hybridises to a probe specific for human U3. We have cloned the genes encoding U1, U2, U3 and U4 from S. pombe, together with that encoding another abundant snRNA, previously designated SPU43. U2 and U4 are encoded by single-copy genes, while two genes encode U3. The latter are not clustered, since a chromosomal Southern transfer shows them to lie on different chromosomes.
粟酒裂殖酵母含有一组五个相对丰富的小核RNA（snRNA），它们可被Sm血清型的人类自身免疫抗体免疫沉淀。粟酒裂殖酵母的RNA与针对人类U1、U2、U4、U5和U6的特异性探针杂交，并且在每种情况下其大小都与人类相应RNA相似。粟酒裂殖酵母的另一组snRNA被抗含U3核糖核蛋白的抗体沉淀；这些种类中最丰富的一种与针对人类U3的特异性探针杂交。我们已经从粟酒裂殖酵母中克隆了编码U1、U2、U3和U4的基因，以及编码另一种丰富的snRNA（先前命名为SPU43）的基因。U2和U4由单拷贝基因编码，而两个基因编码U3。后者并不成簇，因为染色体Southern印迹显示它们位于不同的染色体上。
