Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
J Infect Dis. 2011 Oct 1;204(7):1134-7. doi: 10.1093/infdis/jir461.
The mechanisms underlying the failure to contain the growth of Leishmania parasites in human visceral leishmaniasis (VL) are not understood. L donovani amastigotes were quantified in cultured splenic aspirate cells to assess the function of IL-10 in lesional tissue ex vivo. In 67 patients with active VL, IL-10 neutralization promoted parasite killing in 73% and complete clearance in 30%, while 18% had more parasites and 9% did not change. The splenic cells secreted increased levels of both tumor necrosis factor α (TNFα) and interferon γ (IFNγ) under IL-10-neutralizing conditions. These findings provide direct support for targeting IL-10 as an approach to therapy in human VL.
导致人体内脏利什曼病（VL）中无法控制利什曼原虫生长的机制尚不清楚。通过定量检测培养的脾抽吸细胞中的利什曼原虫无鞭毛体，评估了 IL-10 在病变组织中的功能。在 67 例活动性 VL 患者中，IL-10 中和促进了 73%的寄生虫杀伤和 30%的完全清除，而 18%的患者寄生虫增多，9%的患者寄生虫数量无变化。在 IL-10 中和条件下，脾细胞分泌的肿瘤坏死因子α（TNFα）和干扰素γ（IFNγ）水平增加。这些发现为将 IL-10 作为人类 VL 治疗方法提供了直接支持。
