Yokomuro Hiroki, Shiono Noritsugu, Watanabe Yoshinori, Yoshihara Katsunori, Koyama Nobuya, Okada Mitsumasa
Division of Cardiovascular Surgery, Department of Surgery, School of Medicine, Faculty of Medicine, Toho University, Tokyo, Japan.
Ann Thorac Cardiovasc Surg. 2011;17(5):481-6. doi: 10.5761/atcs.oa.10.01650. Epub 2011 Jul 13.
Tissue engineering with cell seeded biodegradable material has attracted attention as a novel means of treating the severely impaired heart. Here, we consider optimal preparation of a durable biograft using dynamic and static cultures.
Vascular smooth muscle cells (VSMCs) derived from the rat aorta were seeded onto biodegradable material P (LA/CL) (poly-L-lactide-ε-caprolactone copolymer) and cultured as follows: a) Static culture (n = 11), b) dynamic culture (n = 12), c) 0 h pre-seeding (n = 12), d) 24 h pre-seeding (n = 5) and e) 1 week pre-seeding (n = 12). Dynamic culture: Cells were cultured in spinner flasks. Pre-seeding: Static cell seeding and culture before dynamic culture.
The conditions of the P (LA/CL) in the five groups were evaluated as cell proliferation and by histological studies.
VSMCs proliferated both in and on the biodegradable materials. The quality of the dynamic culture cell with pre-seeding increased. Although the duration of pre-seeding exerted no significantly different effects, cell attachment and proliferation were widely scattered in the 0 h pre-seeding group, whereas cells proliferating on the front of the scaffold obstructed proliferation inside the biodegradable material in the 1 week pre-seeding group .
Dynamic cell culture with 24 h pre-seeding is effective for constructing ideal biografts.
